Cellular distribution of transforming growth factor-beta 1 and procollagen types I, III, and IV transcripts in carbon tetrachloride-induced rat liver fibrosis.
Cellular distribution of transforming growth factor-beta 1 and procollagen types I, III, and IV transcripts in carbon tetrachloride-induced rat liver fibrosis.
复制标题
四氯化碳诱导的大鼠肝纤维化中转化生长因子-β1 和 I、III 和 IV 型前胶原转录物的细胞分布。
DOI：
--
复制
发表时间：
1990
影响因子：
15.9
通讯作者：
S. Thorgeirsson
中科院分区：
文献类型：
--
作者：
H. Nakatsukasa;P. Nagy;R. Evarts;C. Hsia;E. Marsden;S. Thorgeirsson
采用原位杂交技术研究了四氯化碳（CCl4）诱导的大鼠肝纤维化中转化生长因子-β 1（TGF-β 1）和前胶原α 1（I）、α 1（III）和α 1（IV）基因转录物的细胞分布和时间表达。在纤维化过程中，TGF-β1和前胶原基因相似地主要在结蛋白阳性的窦周细胞（例如脂肪储存细胞和肌成纤维细胞）和成纤维细胞中表达，并且它们的表达持续高于对照大鼠中观察到的表达。这些转录物也在肝纤维化早期的炎症细胞（主要是粒细胞和巨噬细胞样细胞）中观察到。沿着小血管和纤维隔膜的细胞外基质的产生与这些基因的表达一致。在整个实验过程中，肝细胞中未检测到 TGF-β1 和前胶原基因的表达。未观察到前胶原 α 1(I)、α 1(III) 和 α 1(IV) 之间的细胞分布或基因表达时间进程存在显着差异。结蛋白阳性的窦周细胞和成纤维细胞似乎在 CCl4 诱导的肝纤维化中胶原蛋白的合成中起主要作用。在肝纤维化过程中，间充质细胞（包括结蛋白阳性窦周细胞）中同时表达 TGF-β 1 和前胶原基因，这表明 TGF-β 1 可能在纤维化的产生中发挥重要作用。
The cellular distribution and temporal expression of transcripts from transforming growth factor-beta 1 (TGF-beta 1) and procollagen alpha 1(I), alpha 1(III), and alpha 1(IV) genes were studied in carbon tetrachloride (CCl4)-induced rat liver fibrosis by using in situ hybridization technique. During the fibrotic process, TGF-beta 1 and procollagen genes were similarly and predominantly expressed in Desmin-positive perisinusoidal cells (e.g., fat-storing cells and myofibroblasts) and fibroblasts and their expression continued to be higher than those observed in control rats. These transcripts were also observed in inflammatory cells mainly granulocytes and macrophage-like cells at the early stages of liver fibrosis. The production of extracellular matrix along small blood vessels and fibrous septa coincided with the expression of these genes. Expression of TGF-beta 1 and procollagen genes were not detected in hepatocytes throughout the experiment. No significant differences in cellular distribution or time course of gene expression among procollagen alpha 1(I), alpha 1(III), and alpha 1(IV) were observed. Desmin-positive perisinusoidal cells and fibroblasts appeared to play the principal role in synthesis of collagens in CCl4-induced hepatic fibrosis. The simultaneous expression of TGF-beta 1 and procollagen genes in mesenchymal cells, including Desmin-positive perisinusoidal cells, during hepatic fibrosis suggests the possibility that TGF-beta 1 may have an important role in the production of fibrosis.
登录
查看更多内容
DOI：
--
发表时间：
1986-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Ignotz;J. Massagué
通讯作者：
R. Ignotz;J. Massagué
DOI：
10.1073/pnas.82.24.8681
发表时间：
1985-12-01
影响因子：
11.1
作者：
FRIEDMAN, SL;ROLL, FJ;BISSELL, DM
通讯作者：
BISSELL, DM
DOI：
10.1073/pnas.85.5.1539
发表时间：
1988-03-01
影响因子：
11.1
作者：
BRAUN, L;MEAD, JE;FAUSTO, N
通讯作者：
FAUSTO, N
影响因子：
4.1
作者：
ALAKOKKO, L;PIHLAJANIEMI, T;SAVOLAINEN, ER
通讯作者：
SAVOLAINEN, ER
原代单层培养物和体内肝细胞形成胶原蛋白。
DOI：
10.1126/science.6828863
发表时间：
1983
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Diegelmann,RF;Guzelian,PS;Gay,R;Gay,S
通讯作者：
Gay,S