Using Zebrafish to Elucidate Glial-Vascular Interactions During CNS Development.
Using Zebrafish to Elucidate Glial-Vascular Interactions During CNS Development.
复制标题
DOI：
10.3389/fcell.2021.654338
复制
发表时间：
2021
影响因子：
5.5
通讯作者：
Sontheimer H
中科院分区：
文献类型：
--
作者：
Umans RA;Pollock C;Mills WA 3rd;Clark KC;Pan YA;Sontheimer H
神经科学中的一个新兴领域是神经胶质和血管之间的细胞关系，因为神经胶质的许多假定支持作用需要与血管系统相关联。这些相互作用最好是在体内研究，并取得了很大的进步，使用小鼠纵向图像胶质血管相互作用。然而，这些方法对于发展研究来说是繁琐的，而发展研究可以从更容易获得的系统中受益。斑马鱼（Danio rerio）是遗传上易处理的脊椎动物，并且鉴于其不透明性，很容易适合日常活体成像研究。我们着手研究斑马鱼神经胶质细胞是否具有与哺乳动物神经胶质细胞相互作用并维持发育中的脑血管系统的能力的保守特征。我们利用转基因斑马鱼品系，其中少突胶质细胞转录因子2（olig2）和胶质细胞酸性蛋白（gfap）鉴定了斑马鱼脑中不同的胶质细胞群，并记录了它们与脑血管的对应关系。我们的研究结果表明，olig2+和gfap+斑马鱼神经胶质细胞有不同的谱系，每个相互作用与脑血管先前在小鼠大脑中观察到的。此外，我们通过药理学和遗传学方法操纵这些关系，以区分这些细胞类型在血管发育过程中的作用。寡聚体2+神经胶质细胞在其迁移过程中使用血管作为通路，并且Wnt信号传导抑制降低了它们的单细胞血管共选择。相比之下，在CNS血管生成开始时gfap+神经胶质的消融通过减少血管内皮生长因子（VEGF）损害血管发育，支持gfap+神经胶质在斑马鱼中新脑血管形成期间的作用。这些数据表明，斑马鱼神经胶质细胞，类似于哺乳动物神经胶质细胞，具有不同的谱系，表现出不同的相互作用与血管，是一个合适的模型，用于阐明脊椎动物大脑发育过程中的神经胶质血管的关系。
An emerging area of interest in Neuroscience is the cellular relationship between glia and blood vessels, as many of the presumptive support roles of glia require an association with the vasculature. These interactions are best studied in vivo and great strides have been made using mice to longitudinally image glial-vascular interactions. However, these methods are cumbersome for developmental studies, which could benefit from a more accessible system. Zebrafish (Danio rerio) are genetically tractable vertebrates, and given their translucency, are readily amenable for daily live imaging studies. We set out to examine whether zebrafish glia have conserved traits with mammalian glia regarding their ability to interact with and maintain the developing brain vasculature. We utilized transgenic zebrafish strains in which oligodendrocyte transcription factor 2 (olig2) and glial fibrillary acidic protein (gfap) identify different glial populations in the zebrafish brain and document their corresponding relationship with brain blood vessels. Our results demonstrate that olig2+ and gfap+ zebrafish glia have distinct lineages and each interact with brain vessels as previously observed in mouse brain. Additionally, we manipulated these relationships through pharmacological and genetic approaches to distinguish the roles of these cell types during blood vessel development. olig2+ glia use blood vessels as a pathway during their migration and Wnt signaling inhibition decreases their single-cell vessel co-option. By contrast, the ablation of gfap+ glia at the beginning of CNS angiogenesis impairs vessel development through a reduction in Vascular endothelial growth factor (Vegf), supporting a role for gfap+ glia during new brain vessel formation in zebrafish. This data suggests that zebrafish glia, akin to mammalian glia, have different lineages that show diverse interactions with blood vessels, and are a suitable model for elucidating glial-vascular relationships during vertebrate brain development.
登录
查看更多内容
影响因子：
4.4
作者：
Lange SC;Bak LK;Waagepetersen HS;Schousboe A;Norenberg MD
通讯作者：
Norenberg MD
影响因子：
25
作者：
Chen J;Poskanzer KE;Freeman MR;Monk KR
通讯作者：
Monk KR
影响因子：
7.7
作者：
Matsuoka RL;Marass M;Avdesh A;Helker CS;Maischein HM;Grosse AS;Kaur H;Lawson ND;Herzog W;Stainier DY
通讯作者：
Stainier DY
通过神经胶质调节突触连通性。
影响因子：
64.8
作者：
Eroglu, Cagla;Barres, Ben A.
通讯作者：
Barres, Ben A.
影响因子：
82.9
作者：
Chang J;Mancuso MR;Maier C;Liang X;Yuki K;Yang L;Kwong JW;Wang J;Rao V;Vallon M;Kosinski C;Zhang JJ;Mah AT;Xu L;Li L;Gholamin S;Reyes TF;Li R;Kuhnert F;Han X;Yuan J;Chiou SH;Brettman AD;Daly L;Corney DC;Cheshier SH;Shortliffe LD;Wu X;Snyder M;Chan P;Giffard RG;Chang HY;Andreasson K;Kuo CJ
通讯作者：
Kuo CJ