Aldehyde dehydrogenase 1B1 (ALDH1B1) is a potential biomarker for human colon cancer.
Aldehyde dehydrogenase 1B1 (ALDH1B1) is a potential biomarker for human colon cancer.
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DOI：
10.1016/j.bbrc.2011.01.002
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发表时间：
2011-02-11
影响因子：
3.1
通讯作者：
Vasiliou V
中科院分区：
文献类型：
--
作者：
Chen Y;Orlicky DJ;Matsumoto A;Singh S;Thompson DC;Vasiliou V
乙醛脱氢酶(ALDHs)属于NAD(P)依赖酶超家族，催化内源和外源醛氧化成相应的酸。据报道，ALDHs的表达和/或活性增加，特别是ALDH1A1，在人类癌症中发生。有人认为，ALDH1A1的代谢功能赋予了正常干细胞和癌症干细胞“干性”的特性。然而，在特定类型的人类癌症中，导致ALDH活性增强的ALDH同工酶的特性仍有待阐明。ALDH1B1是一种线粒体ALDH，能代谢多种醛底物，包括乙醛和脂质过氧化产物(LPO)。在本研究中，我们用免疫组织化学方法检测了ALDH1A1和ALDH1B1在人结肠癌(N=40)、肺癌(N=30)、乳腺癌(N=33)和卵巢癌(N=33)中的表达谱。ALDH1A1或ALDH1B1在肿瘤组织中的免疫组织化学表达按强度(1~3级)和广泛性(占总癌细胞的百分比)进行评分。在这里，我们报告了ALDH1B1在癌症组织中的表达分数是ALDH1A1的5.6倍。值得注意的是，40例结肠癌标本中39例ALDH1B1阳性，染色强度为2.8±0.5。我们的研究表明，相对于ALDH1A1，ALDH1B1在本研究中检测的腺癌中表达更深入，并且ALDH1B1在人类结肠腺癌中显著上调，使其成为人类结肠癌的潜在生物标志物。
Aldehyde dehydrogenases (ALDHs) belong to a superfamily of NAD(P)+-dependent enzymes, which catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding acids. Increased expression and/or activity of ALDHs, particularly ALDH1A1, have been reported to occur in human cancers. It is proposed that the metabolic function of ALDH1A1 confers the “stemness” properties to normal and cancer stem cells. Nevertheless, the identity of ALDH isozymes that contribute to the enhanced ALDH activity in specific types of human cancers remains to be elucidated. ALDH1B1 is a mitochondrial ALDH that metabolizes a wide range of aldehyde substrates including acetaldehyde and products of lipid peroxidation (LPO). In the present study, we immunohistochemically examined the expression profile of ALDH1A1 and ALDH1B1 in human adenocarcinomas of colon (N=40), lung (N=30), breast (N=33) and ovary (N=33) using an NIH tissue array. The immunohistochemical expression of ALDH1A1 or ALDH1B1 in tumor tissues was scored by their intensity (scale = 1–3) and extensiveness (% of total cancer cells). Herein we report a 5.6-fold higher expression score for ALDH1B1 in cancerous tissues than that for ALDH1A1. Remarkably, 39 out of 40 colonic cancer specimens were positive for ALDH1B1 with a staining intensity of 2.8 ± 0.5. Our study demonstrates that ALDH1B1 is more profoundly expressed in the adenocarcinomas examined in this study relative to ALDH1A1 and that ALDH1B1 is dramatically upregulated in human colonic adenocarcinoma, making it a potential biomarker for human colon cancer.
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影响因子：
11.2
作者：
Huang EH;Hynes MJ;Zhang T;Ginestier C;Dontu G;Appelman H;Fields JZ;Wicha MS;Boman BM
通讯作者：
Boman BM
影响因子：
5.9
作者：
Vasiliou, V;Pappa, A;Estey, T
通讯作者：
Estey, T
影响因子：
3.2
作者：
Coskran, Timothy M.;Morton, Daniel;Stephenson, Diane T.
通讯作者：
Stephenson, Diane T.
DOI：
10.1073/pnas.0603806103
发表时间：
2006-08-01
影响因子：
11.1
作者：
Chute, John P.;Muramoto, Garrett G.;McDonnell, Donald P.
通讯作者：
McDonnell, Donald P.
影响因子：
3.7
作者：
Deng S;Yang X;Lassus H;Liang S;Kaur S;Ye Q;Li C;Wang LP;Roby KF;Orsulic S;Connolly DC;Zhang Y;Montone K;Bützow R;Coukos G;Zhang L
通讯作者：
Zhang L