Identification of CTX-M Type ESBL E. coli from Sheep and Their Abattoir Environment Using Whole-Genome Sequencing.
Identification of CTX-M Type ESBL E. coli from Sheep and Their Abattoir Environment Using Whole-Genome Sequencing.
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DOI：
10.3390/pathogens10111480
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发表时间：
2021-11-14
期刊：
影响因子：
--
通讯作者：
Cray PJF
中科院分区：
文献类型：
--
作者：
Atlaw NA;Keelara S;Correa M;Foster D;Gebreyes W;Aidara-Kane A;Harden L;Thakur S;Cray PJF
广泛传播的超广谱β-内酰胺酶（ESBL）大肠埃希菌（E。除了关于小型反刍动物的有限信息外，全世界都报告了动物、零售肉类和患者中的大肠杆菌）。本研究对ESBL-E的基因型特征进行了研究。从美国北卡罗来纳州健康绵羊及其屠宰场环境中分离的大肠杆菌。共检出113株ESBL E.对来自绵羊（n = 65）及其屠宰场环境（n = 48）的大肠杆菌分离株进行全基因组测序（WGS）。使用生物信息学工具分析WGS数据。检测到多种CTX-M型β-内酰胺酶基因，即blaCTX-M-1、blaCTX-M-14、blaCTX-M-15、blaCTX-M-27、blaCTX-M-32、blaCTX-M-55和blaCTX-M-65。检测到的其他β-内酰胺酶基因包括blaCMY-2、blaTEM-1A/B/C和blaCAR B-2。此外，确定了对喹诺酮类、氨基糖苷类、非尼考类、四环素类、大环内酯类、林可酰胺类和叶酸途径拮抗剂产生耐药性的抗菌素耐药（AMR）基因和/或点突变。大多数检测到的质粒之间共享的分离物从绵羊和屠宰场环境。序列类型更多地聚集在季节性采样周围，但分散在样品类型之间。总之，我们的研究报告了ESBL E的广泛传播。大肠杆菌在绵羊和屠宰场环境和相关的AMR基因，点突变和质粒。这是第一份关于ESBL E的AMR和WGS综合报告。美国绵羊和屠宰场环境中的大肠杆菌。
Widespread dissemination of extended-spectrum beta-lactamase (ESBL) Escherichia coli (E. coli) in animals, retail meats, and patients has been reported worldwide except for limited information on small ruminants. Our study focused on the genotypic characterization of ESBL E. coli from healthy sheep and their abattoir environment in North Carolina, USA. A total of 113 ESBL E. coli isolates from sheep (n = 65) and their abattoir environment (n = 48) were subjected to whole-genome sequencing (WGS). Bioinformatics tools were used to analyze the WGS data. Multiple CTX-M-type beta-lactamase genes were detected, namely blaCTX-M-1, blaCTX-M-14, blaCTX-M-15, blaCTX-M-27, blaCTX-M-32, blaCTX-M-55, and blaCTX-M-65. Other beta-lactamase genes detected included blaCMY-2, blaTEM-1A/B/C, and blaCARB-2. In addition, antimicrobial resistance (AMR) genes and/or point mutations that confer resistance to quinolones, aminoglycosides, phenicols, tetracyclines, macrolides, lincosamides, and folate-pathway antagonists were identified. The majority of the detected plasmids were shared between isolates from sheep and the abattoir environment. Sequence types were more clustered around seasonal sampling but dispersed across sample types. In conclusion, our study reported wide dissemination of ESBL E. coli in sheep and the abattoir environment and associated AMR genes, point mutations, and plasmids. This is the first comprehensive AMR and WGS report on ESBL E. coli from sheep and abattoir environments in the United States.
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