Combined haploinsufficiency and purifying selection drive retention of RPL36a paralogs in Arabidopsis.
Combined haploinsufficiency and purifying selection drive retention of RPL36a paralogs in Arabidopsis.
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DOI：
10.1038/srep04122
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发表时间：
2014-02-18
影响因子：
4.6
通讯作者：
Micol JL
中科院分区：
文献类型：
--
作者：
Casanova-Sáez R;Candela H;Micol JL
全基因组复制事件在很大程度上推动了被子植物基因组的进化。虽然基因组复制后大部分多余的基因拷贝丢失了，但亚功能化或基因平衡导致了基因拷贝的保留。拟南芥80S核糖体是检验基因平衡假说的一个很好的模型，因为它由80个核糖体蛋白组成，所有这些核糖体蛋白都由属于小基因家族的基因编码。在这里，我们提出了APICULATA2(API2)和RPL36aA同源基因的突变等位基因的分离，这两个基因编码相同的核糖体蛋白，但它们的编码序列有89%的相似性。RPL36aA在野生型中的表达水平高于API2。功能丧失的API2和rpl36aa突变是隐性的，以类似的方式影响叶片的发育。它们与不对称叶片2-1(AS2-1)的双突变体组合导致了叶片极性缺陷，rpl36aa AS2-1比API2 AS2-1更严重。我们的结果强调了结合单倍体不足和纯化选择在保留这些近缘基因在拟南芥基因组中的作用。
Whole-genome duplication events have driven to a large degree the evolution of angiosperm genomes. Although the majority of redundant gene copies after a genome duplication are lost, subfunctionalization or gene balance account for the retention of gene copies. The Arabidopsis 80S ribosome represents an excellent model to test the gene balance hypothesis as it consists of 80 ribosomal proteins, all of them encoded by genes belonging to small gene families. Here, we present the isolation of mutant alleles of the APICULATA2 (API2) and RPL36aA paralogous genes, which encode identical ribosomal proteins but share a similarity of 89% in their coding sequences. RPL36aA was found expressed at a higher level than API2 in the wild type. The loss-of-function api2 and rpl36aa mutations are recessive and affect leaf development in a similar way. Their double mutant combinations with asymmetric leaves2-1 (as2-1) caused leaf polarity defects that were stronger in rpl36aa as2-1 than in api2 as2-1. Our results highlight the role of combined haploinsufficiency and purifying selection in the retention of these paralogous genes in the Arabidopsis genome.
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影响因子：
5.1
作者：
Creff, Audrey;Sormani, Rodnay;Desnos, Thierry
通讯作者：
Desnos, Thierry
影响因子：
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作者：
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通讯作者：
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影响因子：
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Tsukaya, Hirokazu
影响因子：
7.2
作者：
Ito, T;Kim, GT;Shinozaki, K
通讯作者：
Shinozaki, K
影响因子：
7.4
作者：
Chang, IF;Szick-Miranda, K;Bailey-Serres, J
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