Oxymatrine downregulates HPV16E7 expression and inhibits cell proliferation in laryngeal squamous cell carcinoma Hep-2 cells in vitro.
Oxymatrine downregulates HPV16E7 expression and inhibits cell proliferation in laryngeal squamous cell carcinoma Hep-2 cells in vitro.
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羟氨酸下调HPV16E7的表达并抑制喉鳞状细胞癌HEP-2细胞的细胞增殖。
DOI：
10.1155/2015/150390
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发表时间：
2015
影响因子：
--
通讯作者：
Dong P
中科院分区：
文献类型：
--
作者：
Ying XJ;Jin B;Chen XW;Xie J;Xu HM;Dong P
Objective.探讨氧化苦参碱抗喉鳞癌作用的可能机制。方法.本实验观察了氧化苦参碱对喉癌Hep-2细胞增殖、细胞周期时相分布、细胞凋亡及HPV 16 E7基因蛋白和mRNA表达的影响。用siRNA抑制HPV 16 E7表达，以证实下调HPV 16 E7对Hep-2细胞增殖的影响。结果氧化苦参碱对Hep-2细胞的生长和增殖有明显的抑制作用，并呈剂量依赖性和时间依赖性。氧化苦参碱使Hep-2细胞阻滞于G 0/G1期，使G 0/G1期细胞明显增多，S期细胞减少。氧化苦参碱可诱导Hep-2细胞凋亡，7 mg/mL氧化苦参碱作用72 h，细胞凋亡率达42%。Western blotting和逆转录-聚合酶链反应检测显示氧化苦参碱可下调HPV 16 E7基因的表达。HPV 16 E7基因的敲除可有效抑制Hep-2细胞的增殖。结论.苦参素抑制喉癌Hep-2细胞增殖并诱导其凋亡，其机制可能与细胞周期阻滞于G 0/G1期及HPV 16 E7基因表达下调有关。氧化苦参碱被认为是一种可能的预防和治疗喉癌的候选药物。
Objective. To investigate the possible mechanisms of oxymatrine's role in anti laryngeal squamous cell carcinoma. Methods. We examined the effects of oxymatrine on the proliferation, cell cycle phase distribution, apoptosis, and the protein and mRNA expression levels of HPV16E7 gene in laryngeal carcinoma Hep-2 cells in vitro. The HPV16E7 siRNA inhibition was also done to confirm the effect of downregulating HPV16E7 on the proliferation in Hep-2 cells. Results. Oxymatrine significantly inhibited the growth and proliferation of Hep-2 cells in a dose-dependence and time-dependence manner. Oxymatrine blocked Hep-2 cells in G0/G1 phase, resulting in an obvious accumulation of G0/G1 phase cells while decreasing S phase cells. Oxymatrine induced apoptosis of Hep-2 cells, whose apoptotic rate amounted to about 42% after treatment with 7 mg/mL oxymatrine for 72 h. Oxymatrine also downregulated the expression of HPV16E7 gene, as determined by the western blotting and reverse transcription-polymerase chain reaction analysis. Knockdown of HPV16E7 effectively inhibited the proliferation of Hep-2 cells. Conclusions. Oxymatrine inhibits the proliferation and induces apoptosis of laryngeal carcinoma Hep-2 cells, which might be mediated by a significant cell cycle arrest in G0/G1 phase and downregulation of HPV16E7 gene. Oxymatrine is considered to be a likely preventive and curative candidate for laryngeal cancer.
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