Low temperature decreased insecticidal protein contents of cotton and its physiological mechanism.
Low temperature decreased insecticidal protein contents of cotton and its physiological mechanism.
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低温降低棉花杀虫蛋白含量及其生理机制
DOI：
10.3389/fpls.2022.1082926
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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作者：
低温延迟了棉花的生长，增加了生殖器官的脱落，严重降低了品质和产量。此外，由于不利的环境，杀虫剂抗性失败或不稳定的表现也有报道。为研究低温对苏云金芽孢杆菌（Bacillus Thuringenesis, Bt）转基因棉花方形期杀虫蛋白含量的影响，对2个Bt棉花品种思康1号（常规品种SK1）和思康3号（杂交种SK3）施加不同的温度和持续时间。低温胁迫对Bt转基因棉花方形和叶片的杀虫蛋白表达有显著抑制作用，随着温度的降低，杀虫蛋白含量降低了30%。此外，阈值温度导致杀虫蛋白含量显著降低，且随胁迫时间延长呈上升趋势，且降低幅度更大。因此，在方形阶段，低温对Bt蛋白含量的不利影响与低温水平和低温持续时间密切相关。方形Bt蛋白含量与叶片Bt蛋白含量呈正相关，但对低温更为敏感。在整个处理时间内，Bt蛋白水平与丙二醛（MDA）含量、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性呈显著负相关，表明冷诱导氧化应激对Bt蛋白含量有负影响。低温下Bt蛋白含量的降低也与氮素代谢的改变有关。谷草乙酸转氨酶（GOT）和谷氨酸丙酮转氨酶（GPT）活性和可溶性蛋白含量均降低，且随温度的降低幅度更大。游离氨基酸含量、肽酶和蛋白酶活性均随温度的降低而升高，且随温度的降低而升高。这些结果提示，在Bt棉生产中，应警惕棉田期持续24小时以上、低于15-17℃的低温灾害，这可能导致棉田抗性降低，造成严重的经济损失。
Low temperature delayed cotton growth, increased abscission of reproductive organs, and seriously reduced quality and yield. Moreover, failed or unstable performance of insecticidal resistance due to adverse environments have been reported. In order to study the impact of low temperature on the insecticidal protein contents at square stage in Bacillus Thuringenesis (Bt) transgenic cotton, different temperature regimes and durations were imposed on two Bt cotton cultivars, Sikang1 (the conventional cultivar, SK1) and Sikang3 (the hybrid cultivar, SK3). Low temperature stress exhibited a significant inhibitory effect on insecticidal protein expression in squares and leaves of Bt transgenic cotton plants, with insecticidal protein contents decreased up to 30% with decreasing temperature. In addition, the threshold temperature resulting in significant reduction of insecticidal protein contents symbolized a rising trend as stress duration extended, together with a greater reduction observed. Thus, at square stage, the detrimental influence of low temperature on Bt protein contents was closely related to the low temperature level and duration. The square Bt protein content was positively correlated with leaf Bt protein content, but was more sensitive to low temperature. Across the whole treatment duration in both years, square Bt protein level was significantly negatively correlated with malondialdehyde (MDA) contents, as well as the activities of catalase (CAT) and superoxide dismutase (SOD), indicating the negative effect of cold induced oxidative stress on Bt protein contents. The reduced Bt protein contents under low temperature were also related to altered N metabolism. Glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) activities, as well as soluble protein contents in squares reduced, and greater reduction was recorded with decreasing temperature. In contrast, the free amino acid contents, and peptidase and protease activities increased, and greater elevation was noted with decreasing temperature. These results suggested in Bt cotton production, it is necessary to be alert to low temperature disasters that may last for more than 24 hours and lower than 15-17°C during the square stage, which may lead to reduced insecticidal resistance causing serious economic losses.
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影响因子：
4.6
作者：
Ali A;Desneux N;Lu Y;Liu B;Wu K
通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
5.8
作者：
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影响因子：
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作者：
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通讯作者：
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