Micromechanical PDGF recognition via lab-on-a-disc aptasensor arrays.
Micromechanical PDGF recognition via lab-on-a-disc aptasensor arrays.
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DOI：
10.1016/j.sna.2012.06.030
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发表时间：
2013-06-01
影响因子：
4.6
通讯作者：
Boisen, A.
中科院分区：
文献类型：
--
作者：
Bosco, F. G.;Bache, M.;Yang, J.;Chen, C. H.;Hwu, E. -T.;Lin, Q.;Boisen, A.
一个即插即用的类似cd的平台通过多个微悬臂阵列的适配体表面功能化来执行血小板衍生生长因子（PDGF）蛋白的统计检测。当PDGF蛋白与适体涂层结合时，悬臂就会偏转。偏转响应由商用DVD-ROM设备的光学读出单元监测。我们报告了一种改进的传感平台的使用，该平台便于在连续液体流动和温度控制下进行测量。此外，DVD-ROM平台的机械摆动已经最小化，扫描系统已经优化，以便以纳米级分辨率检测液体中的悬臂偏转。通过检测临床相关的PDGF蛋白浓度，证明了该传感平台的能力。我们在不同浓度的PDGF下对100个微悬臂进行了统计测量，范围从10 nM到400 nM。因此，可以可靠地表征悬臂梁的平均力学响应作为蛋白质浓度的函数。
A plug-and-play CD-like platform is used to perform a statistical detection of platelet derived growth factor (PDGF) proteins through aptamer-based surface functionalization of multiple microcantilever arrays. When PDGF proteins bind to aptamer coatings, the cantilevers deflect. The deflection response is monitored by optical read-out units from a commercial DVD-ROM device. We report on the use of an improved sensing platform which facilitates measurements under continuous liquid flow and with temperature control. Also, the mechanical wobbling of the DVD-ROM platform has been minimized and the scanning system has been optimized in order to detect cantilever deflections in liquid with nanometer scale resolution. The capability of the sensing platform is demonstrated by detection of clinically relevant concentrations of PDGF proteins. We present statistical measurements on 100 microcantilevers at different concentrations of PDGF, ranging from 10 nM to 400 nM. Hereby it is possible to reliably characterize the averaged mechanical response of cantilevers as a function of protein concentration.
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影响因子：
6.1
作者：
Bosco, Filippo G.;Hwu, En-Te;Boisen, Anja
通讯作者：
Boisen, Anja
影响因子：
4.6
作者：
Hnilicka, B;Voda, A;Schröder, HJ
通讯作者：
Schröder, HJ
影响因子：
7.4
作者：
Savran, CA;Knudsen, SM;Manalis, SR
通讯作者：
Manalis, SR
影响因子：
3.2
作者：
Vicens, MC;Sen, A;Tan, WH
通讯作者：
Tan, WH
影响因子：
7.4
作者：
Huang, CC;Huang, YF;Chang, HT
通讯作者：
Chang, HT