A short history of pluripotent stem cells markers.
A short history of pluripotent stem cells markers.
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DOI：
10.1016/j.stemcr.2023.11.012
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发表时间：
2024-01-09
影响因子：
5.9
通讯作者：
Gokhale, Paul J.
中科院分区：
文献类型：
--
作者：
Andrews, Peter W.;Gokhale, Paul J.
一种或多种少量分子（通常是细胞表面相关抗原或转录因子）的表达被广泛用于鉴定多能干细胞（PSC）或用于监测其分化。然而，这些标记物分子中没有一个是由PSC唯一表达的，并且全部由已经失去分化能力的干细胞表达。因此，没有一个是多能性的指标。在这里，我们总结了广泛使用的几种标志物的性质和特征，包括细胞表面抗原，阶段特异性胚胎抗原（SSEA）-1，SSEA-3，SSEA-4，TRA-1-60，TRA-1-81，GCTM 2和转录因子POUF 5/OCT 4，NANOG和SOX 2，强调了在解释有关其在推定PSC上表达的数据时必须考虑的问题。本文综述了广泛用于识别和监测PSC的几种标志物的性质和表达模式，强调了在解释推定PSC表达数据时必须考虑的问题。具体而言，它指出没有标记物是由PSC专门表达的，并且没有标记物可以用于明确证明多能性。
The expression of one or more of a small number of molecules, typically cell surface-associated antigens, or transcription factors, is widely used for identifying pluripotent stem cells (PSCs) or for monitoring their differentiation. However, none of these marker molecules are uniquely expressed by PSCs and all are expressed by stem cells that have lost the ability to differentiate. Consequently, none are indicators of pluripotency, per se. Here we summarize the nature and characteristics of several markers that are in wide use, including the cell surface antigens, stage-specific embryonic antigen (SSEA)-1, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81, GCTM2, and the transcription factors POUF5/OCT4, NANOG, and SOX2, highlighting issues that must be considered when interpreting data about their expression on putative PSCs. This review summarizes the nature and expression patterns of several markers widely used for identifying and monitoring PSCs, highlighting issues that must be considered when interpreting data about their expression by putative PSCs. Specifically, it points out that no markers are exclusively expressed by PSCs and none can be used to definitely prove pluripotency.
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影响因子：
5.9
作者：
Allison TF;Smith AJH;Anastassiadis K;Sloane-Stanley J;Biga V;Stavish D;Hackland J;Sabri S;Langerman J;Jones M;Plath K;Coca D;Barbaric I;Gokhale P;Andrews PW
通讯作者：
Andrews PW
DOI：
10.1073/pnas.70.10.2988
发表时间：
1973-01-01
影响因子：
11.1
作者：
ARTZT, K;DUBOIS, P;JACOB, F
通讯作者：
JACOB, F
影响因子：
5.2
作者：
Cantz, Tobias;Key, Goeran;Schoeler, Hans R.
通讯作者：
Schoeler, Hans R.
影响因子：
10.5
作者：
Avilion, AA;Nicolis, SK;Lovell-Badge, R
通讯作者：
Lovell-Badge, R
影响因子：
--
作者：
ANDREWS, PW;BANTING, G;AVNER, P
通讯作者：
AVNER, P