Cloning and molecular characterization of a myosin light chain gene from Puccinia striiformis f. sp. tritici
Cloning and molecular characterization of a myosin light chain gene from Puccinia striiformis f. sp. tritici
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条锈菌肌球蛋白轻链基因的克隆和分子表征。
DOI：
10.1007/s11274-013-1485-z
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发表时间：
2013-09
影响因子：
4.1
通讯作者：
Dan Yu
中科院分区：
文献类型：
--
作者：
Zhensheng Kang;Lina Han;Qiong Zhang;Qiuling Wang;Qing Chang;Hua Zhuang;Jia Liu;Man Li;Dan Yu
小麦条锈菌Puccinia striiformis f.小麦条锈菌（Pst）是一种专性生物营养型担子菌。许多研究发现，肌球蛋白在真菌生长繁殖过程中起着重要作用。然而，关于Pst的肌球蛋白的报道很少。在这项研究中，我们克隆并获得了Pst的肌球蛋白轻链基因PsMLC 1，并表征其表达。此外，PsMLC 1的功能进行了鉴定的突变互补。结果发现，PsMLC 1在裂殖酵母中的表达主要弥补了cdc 4突变体的缺陷，表明PsMLC 1属于肌球蛋白轻链家族成员。表达研究表明，PsMLC 1基因在小麦感染Pst后24 h前转录水平变化不大，随后突然下调。通过使用ML-7，我们观察到PsMLC 1的失活大大降低了夏孢子的萌发率。这些结果表明，PsMLC 1是必不可少的Pst感染小麦的早期阶段，但不必要的感染后期阶段。本研究阐明了Pst中肌球蛋白的功能，为条锈病的防治提供了理论依据。
The fungus Puccinia striiformis f. sp. tritici (Pst), the causal agent of wheat stripe rust, is an obligate biotrophic basidiomycete. Many studies have found that myosins play important roles during fungal growth and propagation. However, there are few reports on the myosins of Pst. In this study, we cloned and obtained the myosin light chain gene PsMLC1 from Pst and characterized its expression. Furthermore, the function of PsMLC1 was identified by mutant complementation. As a result, we found that expression of PsMLC1 in Schizosaccharomyces pombe mostly complemented the defects of the cdc4 mutant, indicating that PsMLC1 belongs to the myosin light chain family member. Expression studies showed that the transcript levels of PsMLC1 little changed before 24 h post inoculation then was suddenly down-regulated during Pst infection of wheat. By using ML-7, we observed that inactivity of PsMLC1 greatly reduced the germination rate of urediniospores. These results suggest that PsMLC1 is essential for the early stages of Pst infection of wheat but unnecessary for the later stages of infection. This work elucidates the function of the myosins in Pst and may provide some theoretical basis for controlling strip rust.
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影响因子：
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作者：
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影响因子：
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DOI：
10.1073/pnas.151262398
发表时间：
2001-07
影响因子：
11.1
作者：
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