Fibrinogen Deposition on Silicone Oil-Infused Silver-Releasing Urinary Catheters Compromises Antibiofilm and Anti-Encrustation Properties.
Fibrinogen Deposition on Silicone Oil-Infused Silver-Releasing Urinary Catheters Compromises Antibiofilm and Anti-Encrustation Properties.
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DOI：
10.1021/acs.langmuir.2c03020
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发表时间：
2023-01-31
期刊：
影响因子：
3.9
通讯作者：
Zhao, Qi
中科院分区：
文献类型：
--
作者：
Zhang, Shuai;Teng, Xiao;Liang, Xinjin;Gadd, Geoffrey Michael;McCoy, Colin Peter;Dong, Yuhang;Wang, Yimeng;Zhao, Qi
最近，可滑动的硅油注入（SOI）表面已成为传统导尿管抗感染涂层的一种有前途的替代品，以对抗生物膜和结壳的形成。受益于超低的迟滞和光滑行为，已经发现液体状SOI涂层在静态和流动条件下都能有效地减少细菌粘附。然而，在真实的临床环境中，导管的使用也可能引发局部炎症，导致宿主分泌的蛋白质的释放，例如沉积在导管表面上的纤维蛋白原（Fgn），产生可被尿路病原体利用以引起感染的小生境。在这项工作中，我们报告了硅油灌注的银释放导管的制造，该导管在水性条件下表现出上级耐久性和强大的抗菌活性，与商业全硅导管相比，7天后两种关键尿路病原体大肠杆菌和奇异变形杆菌的生物膜形成减少了99%，同时对L929小鼠成纤维细胞保持无细胞毒性。暴露于FGN后，油注入表面诱导蛋白质的构象变化，从而加速吸附到表面上。沉积的Fgn阻断了银与细菌的相互作用，并充当支架，这促进了细菌定殖，导致受损的银膜活性。Fgn结合还促进奇异变形杆菌在导管表面上的迁移，并加速结晶生物膜的沉积和扩散。我们的研究结果表明，使用硅油灌注的银释放导尿管可能不是对抗严重炎症引起的感染和相关并发症的可行策略。
Slippery silicone-oil-infused (SOI) surfaces have recently emerged as a promising alternative to conventional anti-infection coatings for urinary catheters to combat biofilm and encrustation formation. Benefiting from the ultralow low hysteresis and slippery behavior, the liquid-like SOI coatings have been found to effectively reduce bacterial adhesion under both static and flow conditions. However, in real clinical settings, the use of catheters may also trigger local inflammation, leading to release of host-secreted proteins, such as fibrinogen (Fgn) that deposits on the catheter surfaces, creating a niche that can be exploited by uropathogens to cause infections. In this work, we report on the fabrication of a silicone oil-infused silver-releasing catheter which exhibited superior durability and robust antibacterial activity in aqueous conditions, reducing biofilm formation of two key uropathogens Escherichia coli and Proteus mirabilis by ∼99%, when compared with commercial all-silicone catheters after 7 days while remaining noncytotoxic toward L929 mouse fibroblasts. After exposure to Fgn, the oil-infused surfaces induced conformational changes in the protein which accelerated adsorption onto the surfaces. The deposited Fgn blocked the interaction of silver with the bacteria and served as a scaffold, which promoted bacterial colonization, resulting in a compromised antibiofilm activity. Fgn binding also facilitated the migration of Proteus mirabilis over the catheter surfaces and accelerated the deposition and spread of crystalline biofilm. Our findings suggest that the use of silicone oil-infused silver-releasing urinary catheters may not be a feasible strategy to combat infections and associated complications arising from severe inflammation.
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光滑的液体注入多孔表面可防止微生物表面污染并杀死周围介质中的非粘附病原体：一种控制释放方法。
影响因子：
19
作者：
Manna U;Raman N;Welsh MA;Zayas-Gonzalez YM;Blackwell HE;Palecek SP;Lynn DM
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DOI：
10.1038/nrmicro3432
发表时间：
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期刊：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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