Amino termini of many yeast proteins map to downstream start codons.
Amino termini of many yeast proteins map to downstream start codons.
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DOI：
10.1021/pr300538f
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发表时间：
2012-12-07
影响因子：
4.4
通讯作者：
Weir MP
中科院分区：
文献类型：
--
作者：
Fournier CT;Cherny JJ;Truncali K;Robbins-Pianka A;Lin MS;Krizanc D;Weir MP
全面了解蛋白质组的复杂性对理解细胞功能至关重要。酵母蛋白的氨基末端通过戊二醛处理的细胞裂解物的肽质谱鉴定，以及对公开沉积的光谱进行平行评估。出乎意料的是，检测到的大部分氨基末端肽（35%）定位于注释起始密码子下游的AUG密码子的翻译起始。许多相关基因在其注释的AUG附近具有翻译起始的次优序列背景，并且它们的核糖体谱显示与下游AUG及其注释的AUG的翻译起始一致的标记密度升高。这些数据表明，酵母蛋白质组的很大一部分来自下游aug的起始，显著增加了编码蛋白的库及其潜在功能和细胞定位。
Comprehensive knowledge of proteome complexity is crucial to understanding cell function. Amino termini of yeast proteins were identified through peptide mass spectrometry on glutaraldehyde-treated cell lysates as well as a parallel assessment of publicly-deposited spectra. An unexpectedly large fraction of detected amino-terminal peptides (35%) mapped to translation initiation at AUG codons downstream of the annotated start codon. Many of the implicated genes have suboptimal sequence contexts for translation initiation near their annotated AUG, and their ribosome profiles show elevated tag densities consistent with translation initiation at downstream AUGs as well as their annotated AUGs. These data suggest that a significant fraction of the yeast proteome derives from initiation at downstream AUGs, increasing significantly the repertoire of encoded proteins and their potential functions and cellular localizations.
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DOI：
10.1083/jcb.108.2.229
发表时间：
1989-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kozak M
通讯作者：
Kozak M
影响因子：
6.8
作者：
Dunker, A. Keith;Silman, Israel;Sussman, Joel L.
通讯作者：
Sussman, Joel L.
影响因子：
7
作者：
Olsen, JV;Ong, SE;Mann, M
通讯作者：
Mann, M
影响因子：
64.8
作者：
Kellis, M;Patterson, N;Lander, ES
通讯作者：
Lander, ES
影响因子：
4.4
作者：
Russo, Allison;Chandramouli, Nagarajan;Deng, Haiteng
通讯作者：
Deng, Haiteng