A comparison of heat-stress transcriptome changes between wild-type Arabidopsis pollen and a heat-sensitive mutant harboring a knockout of cyclic nucleotide-gated cation channel 16 (cngc16).
A comparison of heat-stress transcriptome changes between wild-type Arabidopsis pollen and a heat-sensitive mutant harboring a knockout of cyclic nucleotide-gated cation channel 16 (cngc16).
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DOI：
10.1186/s12864-018-4930-4
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发表时间：
2018-07-24
期刊：
影响因子：
4.4
通讯作者：
Harper JF
中科院分区：
文献类型：
--
作者：
Rahmati Ishka M;Brown E;Weigand C;Tillett RL;Schlauch KA;Miller G;Harper JF
在有花植物中，雄配子体（花粉）是最容易受到温度胁迫的细胞之一。在拟南芥中，花粉特异性的环核苷酸门控阳离子通道16（cngc 16）是温度胁迫条件下植物繁殖所必需的。携带cncg 16基因敲除的植物在炎热的白天和寒冷的夜晚几乎是不育的。为了了解根本原因，使用RNA-Seq来比较正常和热胁迫（HS）条件下野生型（WT）和cngc 16的花粉转录组。在这里，我们发现WT花粉中的热应激反应（HSR）导致2102个具有统计学意义的转录组变化（≥ 2倍变化，调整后的p值≤0.01），约占14，226个定量转录本的15%。在这些变化中，89个对应于转录因子，其中27个在花粉中优先表达。与WT相比，cngc 16花粉显示出1.9倍以上的HS依赖性变化（总共3936个，WT和cngc 16之间有2776个差异）。在WT和cngc 16转录组之间的定量直接比较中，统计学显著差异的数量从正常条件下的21个预先存在的差异增加到HS下的192个差异。在cngc 16中最不同的WT中的20种HS依赖性变化中，一半对应于预测影响细胞壁特征或膜动力学的编码蛋白质的基因。这里的结果定义了大约15%的WT花粉转录组的广泛HS依赖性重编程，并确定了至少27个转录因子的变化，可以提供独特的贡献花粉HSR。WT和cngc 16之间的统计学显著转录组差异的数量在HS下增加了9倍以上，其中大多数最大幅度的变化具有特异性影响细胞壁或膜动力学的潜力，从而增强cngc 16花粉对HS的超敏感性。然而，HS超敏反应也可能是由整个转录组中大量的差异引起的，这些差异对尖端生长和受精所需的多个细胞途径产生累积影响。无论如何，这里的结果支持一个模型，其中花粉转录组的功能性HS依赖性重编程需要特定的钙渗透性环核苷酸门控阳离子通道CNGC 16。本文的在线版本（10.1186/s12864-018-4930-4）包含补充材料，可供授权用户使用。
In flowering plants, the male gametophyte (pollen) is one of the most vulnerable cells to temperature stress. In Arabidopsis thaliana, a pollen-specific Cyclic Nucleotide-Gated cation Channel 16 (cngc16), is required for plant reproduction under temperature-stress conditions. Plants harboring a cncg16 knockout are nearly sterile under conditions of hot days and cold nights. To understand the underlying cause, RNA-Seq was used to compare the pollen transcriptomes of wild type (WT) and cngc16 under normal and heat stress (HS) conditions. Here we show that a heat-stress response (HSR) in WT pollen resulted in 2102 statistically significant transcriptome changes (≥ 2-fold changes with adjusted p-value ≤0.01), representing approximately 15% of 14,226 quantified transcripts. Of these changes, 89 corresponded to transcription factors, with 27 showing a preferential expression in pollen over seedling tissues. In contrast to WT, cngc16 pollen showed 1.9-fold more HS-dependent changes (3936 total, with 2776 differences between WT and cngc16). In a quantitative direct comparison between WT and cngc16 transcriptomes, the number of statistically significant differences increased from 21 pre-existing differences under normal conditions to 192 differences under HS. Of the 20 HS-dependent changes in WT that were most different in cngc16, half corresponded to genes encoding proteins predicted to impact cell wall features or membrane dynamics. Results here define an extensive HS-dependent reprogramming of approximately 15% of the WT pollen transcriptome, and identify at least 27 transcription factor changes that could provide unique contributions to a pollen HSR. The number of statistically significant transcriptome differences between WT and cngc16 increased by more than 9-fold under HS, with most of the largest magnitude changes having the potential to specifically impact cell walls or membrane dynamics, and thereby potentiate cngc16 pollen to be hypersensitive to HS. However, HS-hypersensitivity could also be caused by the extensive number of differences throughout the transcriptome having a cumulative effect on multiple cellular pathways required for tip growth and fertilization. Regardless, results here support a model in which a functional HS-dependent reprogramming of the pollen transcriptome requires a specific calcium-permeable Cyclic Nucleotide-Gated cation Channel, CNGC16. The online version of this article (10.1186/s12864-018-4930-4) contains supplementary material, which is available to authorized users.
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影响因子：
6.9
作者：
Frank G;Pressman E;Ophir R;Althan L;Shaked R;Freedman M;Shen S;Firon N
通讯作者：
Firon N
影响因子：
3.8
作者：
Finka, Andrija;Goloubinoff, Pierre
通讯作者：
Goloubinoff, Pierre
DOI：
10.1093/dnares/dsw051
发表时间：
2017-04-01
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Keller M;Hu Y;Mesihovic A;Fragkostefanakis S;Schleiff E;Simm S
通讯作者：
Simm S
影响因子：
14.9
作者：
Dai X;Zhao PX
通讯作者：
Zhao PX
影响因子：
7.2
作者：
Cheng, Chia-Yi;Krishnakumar, Vivek;Town, Christopher D.
通讯作者：
Town, Christopher D.