Transcriptional profiling of maturing tomato (Solanum lycopersicum L.) microspores reveals the involvement of heat shock proteins, ROS scavengers, hormones, and sugars in the heat stress response.
Transcriptional profiling of maturing tomato (Solanum lycopersicum L.) microspores reveals the involvement of heat shock proteins, ROS scavengers, hormones, and sugars in the heat stress response.
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DOI：
10.1093/jxb/erp234
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发表时间：
2009
影响因子：
6.9
通讯作者：
Firon N
中科院分区：
文献类型：
--
作者：
Frank G;Pressman E;Ophir R;Althan L;Shaked R;Freedman M;Shen S;Firon N
温度过高导致番茄产量下降的主要原因是发育中的花粉粒对高温胁迫高度敏感。高温反应，特别是在植物对高温最敏感的阶段，人们对其了解甚少。为了全面了解热胁迫成熟番茄小孢子HS响应（HSR）的分子机制，利用Affymetrix番茄基因组阵列和dna扩增片段长度多态性（AFLP）技术对热胁迫成熟番茄小孢子进行了详细的转录组学分析。逆转录pcr （RT-PCR）和免疫印迹分析证实了这一结果。获得的数据揭示了小热休克蛋白（HSP）基因家族的特定成员HSP70和HSP90，以及HS转录因子A2 （HSFA2）和HSFA3，以及经典HS应答基因以外的其他因素的参与。结果还表明，HS对活性氧（ROS）清除剂、糖、植物激素和先前与其他类型胁迫有关的调节基因的调节。利用dna - aflp技术，可以检测到番茄Affymetrix芯片中缺失的花粉特异性功能基因，如参与囊泡介导转运和花粉特异性钙依赖性蛋白激酶（CDPK2）的基因。包括LeHSFA2、LeHSP17.4-CII以及LeHSP90和AtVAMP725的同源基因在内的几个基因在cv小孢子中检测到较高的基础表达水平。耐热品种Hazera 3042与小孢子的比较。Hazera 3017（一个热敏品种），将这些基因标记为参与小孢子耐热性的候选基因。这项工作提供了一个全面的分析分子事件背后成熟的小孢子的作物植物，番茄。
Above-optimal temperatures reduce yield in tomato largely because of the high heat stress (HS) sensitivity of the developing pollen grains. The high temperature response, especially at this most HS-sensitive stage of the plant, is poorly understood. To obtain an overview of molecular mechanisms underlying the HS response (HSR) of microspores, a detailed transcriptomic analysis of heat-stressed maturing tomato microspores was carried out using a combination of Affymetrix Tomato Genome Array and cDNA-amplified fragment length polymorphism (AFLP) techniques. The results were corroborated by reverse transcription-PCR (RT-PCR) and immunoblot analyses. The data obtained reveal the involvement of specific members of the small heat shock protein (HSP) gene family, HSP70 and HSP90, in addition to the HS transcription factors A2 (HSFA2) and HSFA3, as well as factors other than the classical HS-responsive genes. The results also indicate HS regulation of reactive oxygen species (ROS) scavengers, sugars, plant hormones, and regulatory genes that were previously implicated in other types of stress. The use of cDNA-AFLP enabled the detection of genes representing pollen-specific functions that are missing from the tomato Affymetrix chip, such as those involved in vesicle-mediated transport and a pollen-specific, calcium-dependent protein kinase (CDPK2). For several genes, including LeHSFA2, LeHSP17.4-CII, as well as homologues of LeHSP90 and AtVAMP725, higher basal expression levels were detected in microspores of cv. Hazera 3042 (a heat-tolerant cultivar) compared with microspores of cv. Hazera 3017 (a heat-sensitive cultivar), marking these genes as candidates for taking part in microspore thermotolerance. This work provides a comprehensive analysis of the molecular events underlying the HSR of maturing microspores of a crop plant, tomato.
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影响因子：
12.3
作者：
Dixon DP;Lapthorn A;Edwards R
通讯作者：
Edwards R
影响因子：
4.3
作者：
Jain, Mukesh;Prasad, P. V. Vara;Chourey, Prem S.
通讯作者：
Chourey, Prem S.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
DOI：
10.1002/9780470988503.ch5
发表时间：
2005-01-01
期刊：
PLANT ABIOTIC STRESS
影响因子：
--
作者：
Larkindale, Jane;Mishkind, Michael;Vierling, Elizabeth
通讯作者：
Vierling, Elizabeth
影响因子：
7.4
作者：
Honys, D;Twell, D
通讯作者：
Twell, D