Proteomic analysis of human serum for finding pathogenic factors and potential biomarkers in preeclampsia.
Proteomic analysis of human serum for finding pathogenic factors and potential biomarkers in preeclampsia.
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DOI：
10.1016/j.placenta.2010.11.007
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发表时间：
2011-02
期刊：
影响因子：
3.8
通讯作者：
Zhang, Z.
中科院分区：
文献类型：
--
作者：
Liu, C.;Zhang, N.;Yu, H.;Chen, Y.;Liang, Y.;Deng, H.;Zhang, Z.
应用一种新的蛋白质组学方法发现子痫前期的潜在致病因素和生物标志物。5例合并先兆子痫患者和5例健康孕妇对照者的血清分别汇总。每个池用肽配体文库珠（PLLBs）处理，通过亲和力去除高丰度蛋白，从而富集低丰度蛋白。洗脱液中的蛋白质采用1D-Gel-LC-MS/MS组合分析。利用光谱计数和提取的离子电流定量蛋白表达水平。本研究在子痫前期鉴定了1172种独特蛋白，在健康对照中鉴定了1149种独特蛋白。51种蛋白在子痫前期和健康孕妇中表达差异，包括绒毛膜促生长激素（CSH）和纤维蛋白-1。31个蛋白表达上调，20个蛋白表达下调。结果表明，肽配体文库结合1D gel-LC-MS/MS分析是鉴定血清中差异表达蛋白的有效方法，补体和凝血激活以及脂质代谢两个生物学过程参与了子痫前期的发病机制。
To apply a novel proteomic method to discover potential pathogenic factors and biomarkers of preeclampsia. Sera from five patients complicated with preeclampsia and five healthy pregnant controls were separately pooled. Each pool was treated with peptide ligand library beads (PLLBs) to remove high abundance proteins by affinity and thus enrich low abundance proteins. The proteins from the eluate were analyzed by a combination of 1D-Gel-LC-MS/MS. Protein expression levels were quantified using spectral counts and the extracted ion current. 1172 unique proteins in preeclampsia and 1149 in healthy controls were identified in the present study. 51 proteins were differentially expressed between preeclampsia and healthy pregnant women including chorionic somatommammptropin hormone (CSH) and fibulin-1. 31 proteins identified were up-regulated and 20 were down-regulated. The results demonstrate that peptide ligand library combining with 1D gel-LC-MS/MS analysis is an efficient method to identify differentially expressed proteins in sera and two biological processes of complement and coagulation activations and lipid metabolism were involved in the pathogenesis of preeclampsia.
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影响因子：
3.8
作者：
Lynch, A. M.;Salmon, J. E.
通讯作者：
Salmon, J. E.
DOI：
10.1016/0002-9378(79)90469-1
发表时间：
1979-01-01
影响因子：
9.8
作者：
POTTER, JM;NESTEL, PJ
通讯作者：
NESTEL, PJ
影响因子：
4.4
作者：
Guerrier, Luc;Claverol, Stephane;Righetti, Pier Giorgio
通讯作者：
Righetti, Pier Giorgio
影响因子：
4.4
作者：
Castagna, A;Cecconi, D;Rigetti, PG
通讯作者：
Rigetti, PG
DOI：
10.1016/0002-9378(89)90665-0
发表时间：
1989-11-01
影响因子：
9.8
作者：
ROBERTS, JM;TAYLOR, RN;MCLAUGHLIN, MK
通讯作者：
MCLAUGHLIN, MK