Masquelet technique: The effect of altering implant material and topography on membrane matrix composition, mechanical and barrier properties in a rat defect model.
Masquelet technique: The effect of altering implant material and topography on membrane matrix composition, mechanical and barrier properties in a rat defect model.
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DOI：
10.1016/j.jbiomech.2018.02.026
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发表时间：
2018-04-27
影响因子：
2.4
通讯作者：
McBride-Gagyi S
中科院分区：
文献类型：
--
作者：
Gaio N;Martino A;Toth Z;Watson JT;Nicolaou D;McBride-Gagyi S
Masquelet技术是一种再生节段性骨缺损的外科手术。两阶段治疗依赖于血管化异物膜的产生，以支持比传统最大值大三倍的骨移植物。从历史上看，该手术一直使用骨水泥垫片来引起膜的产生。然而，膜的形成很容易受到植入物表面性质（如材料和拓扑结构）的影响。本研究旨在确定将垫片材料变更为钛或粗糙化表面光洁度是否会影响膜的机械或屏障性能。对10周龄雄性Sprague道利大鼠给予外部稳定的6 mm股骨缺损，该缺损用预制的骨水泥（PMMA）或钛（TI）垫片填充，垫片表面光滑（~ 1 um）或粗糙（~ 8 um）。植入4周后，收获膜，并评估基质组成、拉伸力学、收缩和屏障功能。使间隔物粗糙化导致显著更顺应的膜。TI间隔物产生更多抑制溶质转运的膜。两组间胶原蛋白或弹性蛋白分布无差异。这表明，不同的膜特性可以通过改变间隔物表面特性来创建。外科医生可能会在不知不觉中通过骨水泥制备技术影响膜形成。
The Masquelet technique is a surgical procedure to regenerate segmental bone defects. The two-phase treatment relies on the production of a vascularized foreign-body membrane to support bone grafts over three times larger than the traditional maximum. Historically, the procedure has always utilized a bone cement spacer to evoke membrane production. However, membrane formation can easily be effected by implant surface properties such as material and topology. This study sought to determine if the membrane’s mechanical or barrier properties are affected by changing the spacer material to titanium or roughening the surface finish. Ten-week-old, male Sprague Dawley rats were given an externally stabilized, 6mm femur defect which was filled with a pre-made spacer of bone cement (PMMA) or titanium (TI) with a smooth (~1um) or roughened (~8um) finish. After 4 weeks of implantation, the membranes were harvested, and the matrix composition, tensile mechanics, shrinkage, and barrier function was assessed. Roughening the spacers resulted in significantly more compliant membranes. TI spacers created membranes that inhibited solute transport more. There were no differences between groups in collagen or elastin distribution. This suggests that different membrane characteristics can be created by altering the spacer surface properties. Surgeons may unknowingly effecting membrane formation via bone cement preparation techniques.
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DOI：
10.5402/2011/290851
发表时间：
2011
期刊：
ISRN orthopedics
影响因子：
--
作者：
Bistolfi A;Massazza G;Verné E;Massè A;Deledda D;Ferraris S;Miola M;Galetto F;Crova M
通讯作者：
Crova M
影响因子：
2.4
作者：
Claes, LE;Heigele, CA
通讯作者：
Heigele, CA
影响因子：
1.7
作者：
Gardner, Michael J.;Putnam, Sara M.;Silva, Matthew J.
通讯作者：
Silva, Matthew J.
DOI：
10.1016/j.injury.2011.03.036
发表时间：
2011-06-01
影响因子：
2.5
作者：
Giannoudis, Peter V.;Faour, Omar;Dimitriou, Rozalia
通讯作者：
Dimitriou, Rozalia
影响因子：
4.1
作者：
Bertram, JEA;Polevoy, Y;Cullinane, DM
通讯作者：
Cullinane, DM