Polysaccharides of Dendrobium officinale Kimura & Migo Leaves Protect Against Ethanol-Induced Gastric Mucosal Injury via the AMPK/mTOR Signaling Pathway in Vitro and vivo.
Polysaccharides of Dendrobium officinale Kimura & Migo Leaves Protect Against Ethanol-Induced Gastric Mucosal Injury via the AMPK/mTOR Signaling Pathway in Vitro and vivo.
复制标题
铁皮石斛多糖
DOI：
10.3389/fphar.2020.526349
复制
发表时间：
2020
影响因子：
5.6
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Ke Y;Zhan L;Lu T;Zhou C;Chen X;Dong Y;Lv G;Chen S
乙醇性胃粘膜损伤是一种常见的胃肠道疾病。从草药中分离的多糖已被证明对乙醇诱导的胃粘膜损伤有效，但铁皮石斛叶多糖（LDOP-1）是否保护粘膜免受乙醇诱导的损伤仍不清楚。因此，本研究在体内外开展了LDOP-1的胃粘膜保护作用及其机制的研究。LDOP-1的化学组成是包含摩尔比为2.0：1.1：0.7：0.5：0.4的甘露糖、半乳糖醛酸、葡萄糖、半乳糖和阿拉伯糖的杂多糖。药理实验结果表明，LDOP-1在体内外均能显著减轻胃黏膜损伤评分和病理损伤，提高抗氧化能力，降低活性氧水平，逆转GES-1的凋亡。研究表明，LDOP-1预处理上调p-AMPK、LC 3 β、HO-1和Beclin-1的表达水平;下调p-mTOR和p62的表达水平;逆转caspase 3、Bax和Bcl-2的表达水平。本研究首次证明LDOP-1可以通过AMPK/mTOR信号通路在体外和体内保护乙醇诱导的胃粘膜损伤。
Ethanol-induced gastric mucosal injury is a common gastrointestinal disorder. Polysaccharides separated from herbs have been shown to be effective for ethanol-induced gastric mucosal injury, but whether the polysaccharides from Dendrobium officinale Kimura & Migo leaves (LDOP-1) protected mucosa from ethanol-induced injury remains unknown. Thus, the present study carried out gastric mucosal protection and the mechanism of LDOP-1 in vivo and vitro. The chemical composition of LDOP-1 was a heteropolysaccharide comprising mannose, galacturonic acid, glucose, galactose, and arabinose at a molar ratio of 2.0:1.1:0.7:0.5:0.4. Pharmacological results showed that LDOP-1 significantly reduced gastric mucosal injury score and pathological injury, improved antioxidant capacity, reduced the level of reactive oxygen species, and reversed the apoptosis of GES-1 in vivo and vitro. Research showed that LDOP-1 pretreatment upregulated the expression level of p-AMPK, LC3β, HO-1, and Beclin-1; downregulated the expression level of p-mTOR and p62; and reversed the expression level of caspase3, Bax, and Bcl-2. This study was the first to demonstrate that LDOP-1 could protect against ethanol-induced gastric mucosal injury via the AMPK/mTOR signaling pathway in vitro and vivo.
登录
查看更多内容
影响因子：
21.3
作者：
通讯作者：
--
影响因子：
--
作者：
Shin IS;Jeon WY;Shin HK;Cha SW;Lee MY
通讯作者：
Lee MY
影响因子：
3.8
作者：
Kwon, Da-Ae;Kim, Yong Sang;Lee, Hak Sung
通讯作者：
Lee, Hak Sung
影响因子：
5.1
作者：
Singh, Abhishek Kumar;Kashyap, Mahendra Pratap;Rizvi, Syed Ibrahim
通讯作者：
Rizvi, Syed Ibrahim
影响因子：
3.5
作者：
Emerling, Brooke M.;Viollet, Benoit;Chandel, Navdeep S.
通讯作者：
Chandel, Navdeep S.