Banhabaekchulchunma-tang, a traditional herbal formula attenuates absolute ethanol-induced gastric injury by enhancing the antioxidant status.
Banhabaekchulchunma-tang, a traditional herbal formula attenuates absolute ethanol-induced gastric injury by enhancing the antioxidant status.
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DOI：
10.1186/1472-6882-13-170
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发表时间：
2013-07-12
影响因子：
--
通讯作者：
Lee MY
中科院分区：
文献类型：
--
作者：
Shin IS;Jeon WY;Shin HK;Cha SW;Lee MY
半夏白术天麻汤（Banhabaekchulchunma-tang）是由14种草药组成的混合物。在中国、日本和韩国，传统上用于治疗贫血、厌食、全身无力和女性不孕症。在这项研究中，我们研究了Banhabaekchulchunma-tang水提取物（BCT）对乙醇诱导的大鼠急性胃损伤的保护作用。通过向每只大鼠灌胃给予5 mL/kg体重的无水乙醇诱导胃损伤。阳性对照组和BCT组在给予无水乙醇前2 h分别经口给予奥美拉唑（50 mg/kg）或BCT（400 mg/kg）。切除每只动物的胃并检查胃粘膜病变。为了确认BCT的保护作用，我们评估了脂质过氧化的程度，还原型谷胱甘肽（GSH）的水平，以及抗氧化酶过氧化氢酶，谷胱甘肽-S-转移酶，谷胱甘肽过氧化物酶和谷胱甘肽还原酶在胃中的活动。此外，我们根据OECD指南进行了急性毒性研究，以评估BCT的安全性。BCT减少乙醇引起的胃粘膜出血、充血和上皮细胞丢失。BCT可降低乙醇诱导的急性胃损伤引起的脂质过氧化反应，增加胃粘膜GSH含量和抗氧化酶活性。此外，BCT在高达5000 mg/kg时未引起任何不良反应。这些结果表明，BCT保护胃粘膜免受乙醇诱导的胃损伤，通过增加抗氧化状态。我们认为，BCT可以开发为一种有效的药物治疗酒精引起的胃损伤。
Banhabaekchulchunma-tang (hange-byakujutsu-tenma-to in Japanese and banxia-baizhu-tianma-tang in Chinese) is a mixture of fourteen herbs. It is used traditionally for the treatment of anemia, anorexia, general weakness, and female infertility in China, Japan, and Korea. In this study, we investigated the protective effects of a Banhabaekchulchunma-tang water extract (BCT) against ethanol-induced acute gastric injury in rats. Gastric injury was induced by intragastric administration of 5 mL/kg body weight of absolute ethanol to each rat. The positive control group and the BCT group were given oral doses of omeprazole (50 mg/kg) or BCT (400 mg/kg), respectively, 2 h prior to the administration of absolute ethanol. The stomach of each animal was excised and examined for gastric mucosal lesions. To confirm the protective effects of BCT, we evaluated the degree of lipid peroxidation, the level of reduced glutathione (GSH), and the activities of the antioxidant enzymes catalase, glutathione-S-transferase, glutathione peroxidase, and glutathione reductase in the stomach. In addition, we conducted an acute toxicity study to evaluate the safety of BCT according to OECD guideline. BCT reduced ethanol-induced hemorrhage, hyperemia, and loss of epithelial cell in the gastric mucosa. BCT reduced the increased lipid peroxidation associated with ethanol-induced acute gastric lesions, and increased the mucosal GSH content and the activities of antioxidant enzymes. In addition, BCT did not cause any adverse effects at up to 5000 mg/kg. These results indicate that BCT protects the gastric mucosa against ethanol-induced gastric injury by increasing the antioxidant status. We suggest that BCT could be developed as an effective drug for the treatment of gastric injury caused by alcohol intake.
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影响因子：
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