Diverse microbiota in palatal radicular groove analyzed by Illumina sequencing: Four case reports.
Diverse microbiota in palatal radicular groove analyzed by Illumina sequencing: Four case reports.
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DOI：
10.12998/wjcc.v9.i23.6846
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发表时间：
2021-08-16
影响因子：
1.1
通讯作者：
Huang DM
中科院分区：
文献类型：
--
作者：
Tan XL;Chen X;Fu YJ;Ye L;Zhang L;Huang DM
腭侧神经根沟是一种罕见的牙齿发育畸形，可作为连接牙周和根尖周炎的通道，但尚无文献分析腭侧神经根沟内的微生物区系。研究对象为4名上颌恒侧切牙伴发牙周牙髓畸形的上颌侧切牙患者。从4例患者的腭侧根沟不同部位采集了12份细菌标本。用Illumina测序分析了腭沟内的分类组成和微生物组结构，得到了1162个可操作的分类单位。大多数样品中以变形杆菌和菲尔米库特菌为主。乳球菌和卟啉单胞菌是杆菌科中一个未知的属，是已知最丰富的属。沟槽三个区段的微生物区系丰富度和多样性没有差异。腭沟内独特的生态位蕴藏着细菌群落，这些细菌群落具有牙髓和牙周感染的一些组成特征。腭沟的存在可能会在根尖和牙颈之间起到相互作用的桥梁作用，从而影响根管治疗和牙周管理等传统治疗方法的疗效。
A palatal radicular groove is an unusual developmental deformity of the tooth, which may serve as a channel linking the periodontal and periapical inflammation, and yet no literature could be obtained analyzing microbiota within the palatal radicular grooves. Four patients diagnosed with palatal radicular groove and concomitant periodontal-endodontic deformity in permanent maxillary lateral incisors were enrolled in this work. Twelve bacterial samples from 4 patients were collected from different parts of the palatal radicular groove during intentional replantation surgery. Illumina sequencing was performed to analyze the taxonomical composition and microbiome structure inside the palatal grooves, and 1162 operational taxonomic units were obtained. The phyla of Firmicutes and Proteobacteria predominated in most of the samples. An unknown genus from the Bacillaceae family, Lactococcus, and Porphyromonas were the most abundant genera identified. There was no difference in the microbiota richness and diversity in three sections of the groove. The unique ecological niches inside the palatal grooves harbored bacterial communities that shared some component features of both the endodontic and periodontal infections. The existence of palatal groove may play an interaction bridge between the root apex and tooth cervix and thus impair the outcome of traditional therapeutic methods such as root canal treatment and periodontal management.
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影响因子：
5
作者：
Sanchez-Sanhueza, G.;Bello-Toledo, H.;Gallardo-Escarate, C.
通讯作者：
Gallardo-Escarate, C.
影响因子：
4.2
作者：
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通讯作者：
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影响因子：
3
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通讯作者：
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作者：
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通讯作者：
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影响因子：
4.2
作者：
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通讯作者：
Paster BJ