Microbiomes of Endodontic-Periodontal Lesions before and after Chemomechanical Preparation.
Microbiomes of Endodontic-Periodontal Lesions before and after Chemomechanical Preparation.
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DOI：
10.1016/j.joen.2015.08.022
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发表时间：
2015-12
影响因子：
4.2
通讯作者：
Paster BJ
中科院分区：
文献类型：
--
作者：
Gomes BP;Berber VB;Kokaras AS;Chen T;Paster BJ
本研究旨在评估化学机械预备（CMP）前后牙髓-牙周病变（EPL）的微生物群落。临床标本取自15个根管（RC）与坏死的牙髓组织，并从其相关的牙周袋（PP，n=15）的牙齿与EPL前和CMP后。使用下一代测序的人类口腔微生物鉴定（HOMINGS）方案和活菌培养物分析来自RC和PP的样品。进行Mann-Whitney检验和Benjamini-Hochberg校正以将临床和放射学发现与微生物发现相关联（p<0.05）。使用NGS在两个研究中心的100%样本（15/15）中检出细菌。厚壁菌门是最主要的门在这两个网站使用这两种方法。CMP前后NGS法在RC中最常检出的菌种为粪肠球菌、微小微单胞菌、艰难分枝杆菌、Filifactoralocis和Fretibacteriumfastidiosum。在CMP前后，NGS在PP中最常检测到的物种是P. micra、E. faecalis、星座链球菌（Streptococcus constellatus）、短真杆菌（Eubacterium brachyB.）alocis。根尖周病变≤ 2 mm与Desulfobulbus sp. oral taxon 041之间存在相关性;牙周袋≥ 6 mm与Dialister invisius和Peptostrectomatis之间存在相关性（所有p<0.05，在CMP前的RC中发现）。它的结论是，微生物群落存在于联合牙髓牙周病变是复杂的，比以前报道的更多样化。值得注意的是，化学机械预备后，细菌确实在某些根管中存活。最后，化学机械制备前后两个部位的微生物群之间的相似性表明，牙髓和牙周组织之间可能存在感染途径。
This study was conducted to evaluate the microbiomes of endodontic- periodontal lesions (EPL) before and after chemomechanical preparation (CMP). Clinical samples were taken from 15 root canals (RC) with necrotic pulp tissues and from their associated periodontal pockets (PP, n=15) of teeth with EPL before and after CMP. The Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS) protocol and viable culture were used to analyze samples from RC and PP. The Mann-Whitney test and Benjamini-Hochberg corrections were performed to correlate the clinical and radiographic findings with microbial findings (p<0.05). Bacteria were detected in 100% of the samples in both sites (15/15) using NGS. Firmicutes was the most predominant phylum in both sites using both methods. The most frequently detected species in the RC before and after CMP by NGS were Enterococcus faecalis, Parvimonas micra, Mogibacterium timidum, Filifactor alocis, and Fretibacterium fastidiosum. Species most frequently detected in the PP before and after CMP by NGS were P. micra, E. faecalis, Streptococcus constellatus, Eubacterium brachy, Tannerella forsythia and F. alocis. Associations were found between periapical lesions ≤ 2 mm and Desulfobulbus sp. oral taxon 041; and with periodontal pockets ≥ 6mm and Dialister invisius and Peptostreptococcus stomatis (all p<0.05, found in the RC before CMP).). It is concluded that the microbial community present in combined endodontic-periodontal lesions is complex and more diverse than previously reported. It is important to note that bacteria do survive in some root canals after chemomechanical preparation. Finally, the similarity between the microbiota of both sites, before and after chemomechanical preparation, suggests there may be a pathway of infection between the pulp and periodontium.
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影响因子：
3.7
作者：
Santos AL;Siqueira JF Jr;Rôças IN;Jesus EC;Rosado AS;Tiedje JM
通讯作者：
Tiedje JM
影响因子：
4.4
作者：
Hsiao WW;Li KL;Liu Z;Jones C;Fraser-Liggett CM;Fouad AF
通讯作者：
Fouad AF
影响因子：
5
作者：
Didilescu, A. C.;Rusu, D.;Stratul, S. I.
通讯作者：
Stratul, S. I.
影响因子：
3.7
作者：
Gonçalves PF;Klepac-Ceraj V;Huang H;Paster BJ;Aukhil I;Wallet SM;Shaddox LM
通讯作者：
Shaddox LM
影响因子：
4.2
作者：
Gomes, Brenda P. F. A.;Martinho, Frederico C.;Vianna, Morgana E.
通讯作者：
Vianna, Morgana E.