Antibody conjugated magnetic iron oxide nanoparticles for cancer cell separation in fresh whole blood.
Antibody conjugated magnetic iron oxide nanoparticles for cancer cell separation in fresh whole blood.
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DOI：
10.1016/j.biomaterials.2011.08.076
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发表时间：
2011-12
期刊：
影响因子：
14
通讯作者：
Wang, Andrew
中科院分区：
文献类型：
--
作者：
Xu, Hengyi;Aguilar, Zoraida P.;Yang, Lily;Kuang, Min;Duan, Hongwei;Xiong, Yonghua;Wei, Hua;Wang, Andrew
开发了一种使用基于氧化铁磁性纳米颗粒（IO）的免疫磁性分离新鲜全血中肿瘤细胞的高效方法。该工艺涉及聚合物包被的30 nm IO，其用抗人上皮生长因子受体2（抗HER 2或抗HER 2/neu）的抗体（Ab）修饰，形成IO-Ab。HER 2是一种细胞膜蛋白，在几种类型的人类癌细胞中过度表达。使用HER 2/neu过表达人乳腺癌细胞系SK-BR 3作为模型细胞，IO-Ab用于分离73.6%（最大捕获率为84%）的SK-BR 3细胞，这些细胞加标在1 mL新鲜人全血中。IO-Ab优先结合SK-BR 3细胞而不是血液中发现的正常细胞，这是因为癌细胞上的HER 2/neu受体水平高，与正常细胞表面不同。结果表明，纳米尺寸的磁性纳米颗粒在磁场（梯度为100 T/m）中表现出1：10，000，000的富集因子（癌细胞相对于正常细胞），这是通过IO-Ab结合在细胞表面导致对癌细胞的优先捕获。这项研究有望在新鲜全血中有效分离循环癌细胞。
A highly efficient process using iron oxide magnetic nanoparticles (IO)-based immunomagnetic separation of tumor cells from fresh whole blood has been developed. The process involved polymer coated 30 nm IO that was modified with antibodies (Ab) against human epithelial growth factor receptor 2 (anti-HER2 or anti-HER2/neu) forming IO-Ab. HER2 is a cell membrane protein that is over expressed in several types of human cancer cells. Using a HER2/neu over expressing human breast cancer cell line, SK-BR3, as a model cell, the IO-Ab was used to separate 73.6 % (with a maximum capture of 84%) of SK-BR3 cells that were spiked in 1 mL of fresh human whole blood. The IO-Ab preferentially bound to SK-BR3 cells over normal cells found in blood due to the high level of HER2/neu receptor on the cancer cells unlike the normal cell surfaces. The results showed that the nanosized magnetic nanoparticles exhibited an enrichment factor (cancer cells over normal cells) of 1:10,000,000 in a magnetic field (with gradient of 100 T/m) through the binding of IO-Ab on the cell surface that resulted in the preferential capture of the cancer cells. This research holds promise for efficient separation of circulating cancer cells in fresh whole blood.
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