SERS-based immunomagnetic bead for rapid detection of H5N1 influenza virus.

SERS-based immunomagnetic bead for rapid detection of H5N1 influenza virus.
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DOI:
10.1111/irv.13114
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发表时间:
2023-03
影响因子:
4.4
通讯作者:
Li, Qianxue
Li, Qianxue
中科院分区:
医学4区
文献类型:
--
作者:
Wang, Xiwen;Li, Song;Qu, Han;Hao, Liangyu;Shao, Ting;Wang, Kai;Xia, Zhiping;Li, Zhiping;Li, Qianxue
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表面增强拉曼散射(Sers)技术在呼吸道病毒检测中的应用近年来引起了人们的关注,但直接检测病毒的报道还很少。本研究建立了一种夹心免疫磁珠Sers,用于H5 N1流感病毒的快速诊断。检测限估计为5.0 × 10−6 TCID 50/ml。该方法具有良好的特异性,与H1N1、H5 N6或H9 N2无交叉反应。该方法对鸡胚中H5 N1流感病毒的检测准确率为100%。这些结果为利用Sers作为流感病毒诊断的创新方法提供了很大的希望。
The surface‐enhanced Raman scattering (SERS) has recently drawn attention in the detection of respiratory viruses, but there have been few reports of the direct detection of viruses. In this study, a sandwich immunomagnetic bead SERS was established for the rapid diagnosis of the H5N1 influenza virus. The detection limit was estimated to be 5.0 × 10−6 TCID50/ml. The method showed excellent specificity with no cross‐reaction with H1N1, H5N6 or H9N2. The H5N1 influenza virus detection accuracy of the SERS method was 100% in chicken embryos. The results hold great promise for the utilization of SERS as an innovative approach in the diagnosis of influenza virus.
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