Interferometer-based structured-illumination microscopy utilizing complementary phase relationship through constructive and destructive image detection by two cameras.
Interferometer-based structured-illumination microscopy utilizing complementary phase relationship through constructive and destructive image detection by two cameras.
复制标题
基于干涉仪的结构照明显微镜通过两个相机进行相长和相消图像检测，利用互补相位关系。
DOI：
10.1111/j.1365-2818.2012.03604.x
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发表时间：
2012-06
影响因子：
2
通讯作者：
Sedat JW
中科院分区：
文献类型：
--
作者：
Shao L;Winoto L;Agard DA;Gustafsson MG;Sedat JW
在基于干涉仪的荧光显微镜中，通常使用分束器以干涉方式将两个发射波前联合收割机组合。干涉条纹可以沿着两条垂直的路径沿着传播，并且通常仅一条用于成像。然而，另一条路径也包含有用的信息。在这里，我们在基于干涉仪的三维结构照明显微镜（I5S）中引入了第二个相机，以捕获沿着正常未使用路径的条纹，这些条纹相对于沿着另一路径的条纹相位相差π。基于这种互补相位关系和I5S数据分量之间定义良好的相位相互关系，我们可以使用沿两个成像路径沿着同时记录的条纹来推导并且然后计算地消除干涉仪环路内的路径长度误差。这种自校正能力可以极大地放松在每个成像会话之前消除路径长度差异并在每个成像会话期间保持该状态的要求，这实际上是具有挑战性的任务。实验数据表明，支持理论。
In an interferometer-based fluorescence microscope, a beam splitter is often used to combine two emission wavefronts interferometrically. There are two perpendicular paths along which the interference fringes can propagate and normally only one is used for imaging. However, the other path also contains useful information. Here we introduced a second camera to our interferometer-based three-dimensional structured-illumination microscope (I5S) to capture the fringes along the normally unused path, which are out of phase by π relative to the fringes along the other path. Based on this complementary phase relationship and the well-defined phase interrelationships among the I5S data components, we can deduce and then computationally eliminate the path length errors within the interferometer loop using the simultaneously recorded fringes along the two imaging paths. This self-correction capability can greatly relax the requirement for eliminating the path length differences before and maintaining that status during each imaging session, which are practically challenging tasks. Experimental data is shown to support the theory.
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影响因子：
3.4
作者：
Shao, Lin;Isaac, Berith;Gustafsson, Mats G. L.
通讯作者：
Gustafsson, Mats G. L.
影响因子：
2
作者：
Gustafsson, MGL;Agard, DA;Sedat, JW
通讯作者：
Sedat, JW
影响因子：
3.4
作者：
Gustafsson, Mats G. L.;Shao, Lin;Sedat, John W.
通讯作者：
Sedat, John W.
DOI：
10.1364/josaa.9.002159
发表时间：
1992-12-01
影响因子：
1.9
作者：
HELL, S;STELZER, EHK
通讯作者：
STELZER, EHK
影响因子：
2
作者：
Gustafsson, MGL
通讯作者：
Gustafsson, MGL