Monoclonal antibodies to glycoprotein B differentiate human herpesvirus 6 into two clusters, variants A and B.
Monoclonal antibodies to glycoprotein B differentiate human herpesvirus 6 into two clusters, variants A and B.
复制标题
糖蛋白 B 的单克隆抗体将人疱疹病毒 6 分为两个簇：变种 A 和 B。
作者：
G. Campadelli;S. Guerrini;X. Liu;L. Foá
用U1102感染的淋巴细胞免疫小鼠，制备了一组单克隆抗体（MAb），对不同株人疱疹病毒6型（HHV-6）糖蛋白B（gB）的分布进行了分析。MAB 2D 9与U1102和GS分离株发生特异性免疫荧光和免疫沉淀反应，而与Z29和变异株B Hashimoto和SF不发生反应。此外，Z29、Hashimoto和SF gB的M（r）低于U1102和GS gB。MAb 2D 9也未能与皮疹亚急性分离株CV反应，该分离株作为尚未充分表征的分离株纳入本研究。与该结果一致，CV不能与针对gp 82 -105的变异体A特异性MA B b反应，并且表现为变异体B病毒，即使对于位于与pZVH 14探针杂交的片段中的诊断性HindIII内切核酸酶限制性切割位点也是如此。与单克隆抗体2D 9相比，单克隆抗体2B 9和2D 10与所有供试分离株均发生反应，进一步证实了它们具有共同的表位。根据gB的抗原特异性和M（r）特异性，将检测的HHV-6分离株分为两个不重叠的簇，这两个簇与先前通过几个标准（包括限制性内切酶多态性、抗原变异、体外培养生长和序列分析）定义的变异A和B株组密切平行。
The distribution of glycoprotein B (gB) among different human herpesvirus 6 (HHV-6) strains was analysed with a panel of three monoclonal antibodies (MAbs) derived from mice immunized with U1102-infected lymphocytes. MAb 2D9 reacted specifically by immunofluorescence and immunoprecipitation with the U1102 and GS isolates, and failed to react with Z29 and the variant B strains Hashimoto and SF. In addition, Z29, Hashimoto and SF gB had a lower M(r) than U1102 and GS gB. MAb 2D9 also failed to react with the exanthem subitum isolate CV, included in this study as an as yet poorly characterized isolate. Consistent with this result, CV failed to react with the variant A-specific MAb to gp82-105 and behaved as a variant B virus even with respect to the diagnostic HindIII endonuclease restriction cleavage site located in a fragment hybridizing to the pZVH14 probe. By contrast with MAb 2D9, MAbs 2B9 and 2D10 reacted with all of the isolates tested, strengthening the argument tha they have common epitopes. Based on the antigenic and M(r) specificities of gB, the HHV-6 isolates tested were arranged into two non-overlapping clusters, which closely parallel the variant A and B strain groups, defined previously by several criteria, including restriction endonuclease polymorphism, antigenic variations, growth in in vitro cultures and sequence analyses.
人类疱疹病毒 6 株复制和抗原特性的变化。
DOI：
10.1093/infdis/162.4.852
发表时间：
1990
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Wyatt,LS;Balachandran,N;Frenkel,N
通讯作者：
Frenkel,N
来自北美婴儿的人类疱疹病毒 6 分离株的表型和遗传多态性。
影响因子：
3.7
作者：
Dewhurst,S;Chandran,B;McIntyre,K;Schnabel,K;Hall,CB
通讯作者：
Hall,CB
影响因子：
3.7
作者：
ABLASHI, DV;BALACHANDRAN, N;GALLO, RC
通讯作者：
GALLO, RC