Exhaled SARS-CoV-2 RNA viral load kinetics measured by facemask sampling associates with household transmission.
Exhaled SARS-CoV-2 RNA viral load kinetics measured by facemask sampling associates with household transmission.
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DOI：
10.1016/j.cmi.2022.07.005
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发表时间：
2023-03
影响因子：
14.2
通讯作者：
Barer, Michael R.
中科院分区：
文献类型：
--
作者：
Pan, Daniel;Williams, Caroline M.;Decker, Jonathan;Fletcher, Eve;Sze, Shirley;Assadi, Sara;Haigh, Richard;Saleem, Baber;Nazareth, Joshua;Garton, Natalie J.;Pareek, Manish;Barer, Michael R.
关键词：
没有研究检查急性感染期间自然呼出的SARS-CoV-2 RNA病毒载量（VL）的纵向模式。我们使用面罩取样（FMS）报告了这一情况，并评估了发射的RNA VL与家庭传播之间的关系。在2020年12月至2021年2月期间，我们招募了上呼吸道采样（URTS） RT-qPCR阳性24小时内（第0天）的参与者。直到第21天，参与者进行了7次FMS（1小时）和URTS（自摄）。采用RT-qPCR对样本进行分析（来自口罩内的取样基质条），并记录症状日记。通过报告家庭接触者中URTS RT-qPCR阳性来评估家庭传播。来自34名参与者的203个FMS和190个URTS分析表明，RNA VL在采样后的前5天内达到峰值。然而，伴随的URTS， FMS RNA VL和症状评分相关性较差，但报告的症状严重程度较高与FMS阳性相关，直至第5天。在有家庭接触者的28名参与者中，有12人（43%）报告了传播。家庭传播频率与获得的最高（峰值）FMS RNA VL相关（负基因组拷贝/条：0%家庭传播；1至1000拷贝/条：20%；1001至10000拷贝/条：57%；bbb10 000拷贝/条：75%；p = 0.048；年龄调整后家庭传播每对数拷贝/条增加的OR: 4.97; 95% CI, 1.20-20.55; p = 0.02），但与URTS RNA VL峰值无关。FMS测量的呼出RNA VL在早期感染中最高，在伴有URTS阴性的有症状患者中可能呈阳性，并且与家庭传播密切相关。
No studies have examined longitudinal patterns of naturally exhaled SARS-CoV-2 RNA viral load (VL) during acute infection. We report this using facemask sampling (FMS) and assessed the relationship between emitted RNA VL and household transmission. Between December 2020 and February 2021, we recruited participants within 24 hours of a positive RT-qPCR on upper respiratory tract sampling (URTS) (day 0). Participants gave FMS (for 1 hour) and URTS (self-taken) on seven occasions up to day 21. Samples were analysed by RT-qPCR (from sampling matrix strips within the mask) and symptom diaries were recorded. Household transmission was assessed through reporting of positive URTS RT-qPCR in household contacts. Analysis of 203 FMS and 190 URTS from 34 participants showed that RNA VL peaked within the first 5 days following sampling. Concomitant URTS, FMS RNA VL, and symptom scores, however, were poorly correlated, but a higher severity of reported symptoms was associated with FMS positivity up to day 5. Of 28 participants who had household contacts, 12 (43%) reported transmission. Frequency of household transmission was associated with the highest (peak) FMS RNA VL obtained (negative genome copies/strip: 0% household transmission; 1 to 1000 copies/strip: 20%; 1001 to 10 000 copies/strip: 57%; >10 000 copies/strip: 75%; p = 0.048; age adjusted OR of household transmission per log increase in copies/strip: 4.97; 95% CI, 1.20–20.55; p = 0.02) but not observed with peak URTS RNA VL. Exhaled RNA VL measured by FMS is highest in early infection, can be positive in symptomatic patients with concomitantly negative URTS, and is strongly associated with household transmission.
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DOI：
10.1093/cid/ciab797
发表时间：
2022-08-24
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
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作者：
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发表时间：
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影响因子：
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作者：
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影响因子：
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10.1080/02786826.2012.762973
发表时间：
2013-01-01
期刊：
Aerosol science and technology : the journal of the American Association for Aerosol Research
影响因子：
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发表时间：
2022-08-24
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
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