Extraembryonic Endoderm (XEN) Cells Capable of Contributing to Embryonic Chimeras Established from Pig Embryos.
Extraembryonic Endoderm (XEN) Cells Capable of Contributing to Embryonic Chimeras Established from Pig Embryos.
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DOI：
10.1016/j.stemcr.2020.11.011
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发表时间：
2021-01-12
影响因子：
5.9
通讯作者：
Telugu BP
中科院分区：
文献类型：
--
作者：
Park CH;Jeoung YH;Uh KJ;Park KE;Bridge J;Powell A;Li J;Pence L;Zhang L;Liu T;Sun HX;Gu Y;Shen Y;Wu J;Izpisua Belmonte JC;Telugu BP
我们目前关于早期谱系规范和胚胎来源干细胞的大部分知识来自啮齿动物模型的研究。然而，我们对这些非啮齿动物物种的发育过程的理解仍然存在关键差距。本文报道了猪胚外胚层（pXEN）细胞的详细特征，该细胞可以可靠地、可重复地从囊胚的原始胚层（PrE）中产生。高度可扩展的pXEN细胞表达典型的PrE标记物，转录类似于啮齿动物的xen。pXEN细胞在注入宿主囊胚后，可形成内脏卵黄囊和胚胎肠等胚胎外组织，并在体细胞核移植（SCNT）中作为核供体产生活的后代。pXEN细胞系为研究谱系分离提供了一种新的模型，也为家畜基因组编辑提供了一个来源。原始内胚层（PrE）是猪胚泡发育的主要细胞系pXEN细胞具有前祖细胞和类似啮齿动物的XEN细胞的关键特征pXEN细胞在体内可形成胚外和胚胎（肠道）内胚层pXEN细胞可通过体细胞核移植支持足月发育。在这篇文章中，Telugu， Park及其同事表明，原始内胚层是培养中猪胚发育的主要细胞系。可靠地、可重复地建立胚胎外内胚层（XEN）细胞。在嵌合体中，pXEN细胞对胚胎和胚胎外内胚层都有贡献，并通过体细胞核移植支持后代的发育。
Most of our current knowledge regarding early lineage specification and embryo-derived stem cells comes from studies in rodent models. However, key gaps remain in our understanding of these developmental processes from nonrodent species. Here, we report the detailed characterization of pig extraembryonic endoderm (pXEN) cells, which can be reliably and reproducibly generated from primitive endoderm (PrE) of blastocyst. Highly expandable pXEN cells express canonical PrE markers and transcriptionally resemble rodent XENs. The pXEN cells contribute both to extraembryonic tissues including visceral yolk sac as well as embryonic gut when injected into host blastocysts, and generate live offspring when used as a nuclear donor in somatic cell nuclear transfer (SCNT). The pXEN cell lines provide a novel model for studying lineage segregation, as well as a source for genome editing in livestock. Primitive endoderm (PrE) is the predominant lineage emerging from pig blastocyst outgrowths pXEN cells exhibit key features of PrE-progenitors and resemble rodent XEN cells pXEN cells contribute to extraembryonic and embryonic (gut) endoderm in vivo pXEN cells can support full-term development via somatic cell nuclear transfer In this article, Telugu, Park, and colleagues show that primitive endoderm is the predominant lineage emerging from porcine (p) embryonic outgrowths in culture, and reliably and reproducibly establish extraembryonic endoderm (XEN) cells. The pXEN cells were shown to contribute to both embryonic and extraembryonic endoderm in chimeras, and support development of live offspring via somatic cell nuclear transfer.
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