Missense Mutation in the PAS2 Domain of Phytochrome A Impairs Subnuclear Localization and a Subset of Responses Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.000521.
Missense Mutation in the PAS2 Domain of Phytochrome A Impairs Subnuclear Localization and a Subset of Responses Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.000521.
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光敏色素 A 的 PAS2 结构域中的错义突变损害亚核定位和响应子集文章、出版日期和引文信息可在 www.plantcell.org/cgi/doi/10.1105/tpc.000521 上找到。
DOI：
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
J. Casal
中科院分区：
文献类型：
--
作者：
M. Yanovsky;J. Luppi;D. Kirchbauer;O. Ogorodnikova;V. Sineshchekov;É. Ádám;S. Kircher;R. Staneloni;E. Schäfer;F. Nagy;J. Casal
光敏色素A信号显示两个光生物学离散输出：所谓的极低通量响应（VLFR）和高辐照度响应（HIR）。通过修改先前的筛选方案，我们分离出两个保留VLFR和缺乏HIR的拟南芥突变体。光敏色素A负或正调节光敏色素B信号，这取决于光照条件。这些突变体保留了负调控，但缺乏正调控。这两个突变体都携带新的phyA-302等位基因，其中Glu-777（在被子植物光敏色素中保守的残基）在c端结构域的PAS2基序中变为Lys。phyA-302突变体在黑暗中光敏色素a水平降低了50%，但这种差异在连续远红光下的稳定性得到了补偿。phyA-302：绿色荧光蛋白融合蛋白在连续远红光下从胞浆向细胞核正常易位，但不能产生核斑点，提示核斑点可能参与HIR信号通路和光敏色素A降解。我们认为光敏色素A的PAS2结构域在HIR中是启动信号所必需的，但在VLFR中不是，可能通过与特定伙伴的相互作用。
Phytochrome A signaling shows two photobiologically discrete outputs: so-called very-low-fluence responses (VLFR) and high-irradiance responses (HIR). By modifying previous screening protocols, we isolated two Arabidopsis mutants retaining VLFR and lacking HIR. Phytochrome A negatively or positively regulates phytochrome B signaling, depending on light conditions. These mutants retained the negative but lacked the positive regulation. Both mutants carry the novel phyA-302 allele, in which Glu-777 (a residue conserved in angiosperm phytochromes) changed to Lys in the PAS2 motif of the C-terminal domain. The phyA-302 mutants showed a 50% reduction in phytochrome A levels in darkness, but this difference was compensated for by greater stability under continuous far-red light. phyA-302:green fluorescent protein fusion proteins showed normal translocation from the cytosol to the nucleus under continuous far-red light but failed to produce nuclear spots, suggesting that nuclear speckles could be involved in HIR signaling and phytochrome A degradation. We propose that the PAS2 domain of phytochrome A is necessary to initiate signaling in HIR but not in VLFR, likely via interaction with a specific partner.
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影响因子：
11.6
作者：
PARKS, BM;QUAIL, PH
通讯作者：
QUAIL, PH
DOI：
10.1105/tpc.7.9.1433
发表时间：
1995-09
期刊：
The Plant cell
影响因子：
--
作者：
Yong Xu;B. Parks;T. W. Short;P. Quail
通讯作者：
Yong Xu;B. Parks;T. W. Short;P. Quail
影响因子：
56.9
作者：
Fankhauser, C;Yeh, KC;Chory, J
通讯作者：
Chory, J
DOI：
10.1073/pnas.140520097
发表时间：
2000-07-05
影响因子：
11.1
作者：
Krall, L;Reed, JW
通讯作者：
Reed, JW
DOI：
10.1073/pnas.95.23.13976
发表时间：
1998-11-10
影响因子：
11.1
作者：
Yeh, KC;Lagarias, JC
通讯作者：
Lagarias, JC