Uncoupled mitochondria quickly shorten along their long axis to form indented spheroids, instead of rings, in a fission-independent manner.
Uncoupled mitochondria quickly shorten along their long axis to form indented spheroids, instead of rings, in a fission-independent manner.
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DOI：
10.1038/s41598-017-18582-6
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发表时间：
2018-01-10
影响因子：
4.6
通讯作者：
Ohta K
中科院分区：
文献类型：
--
作者：
Miyazono Y;Hirashima S;Ishihara N;Kusukawa J;Nakamura KI;Ohta K
线粒体膜电位(ΔΨm)的丧失会引发线粒体从管状到球状的戏剧性结构变化，称为线粒体碎裂；由此产生的球状线粒体称为肿胀或环状/环状线粒体。我们用一种新发展的相关显微方法结合荧光显微镜、活体成像和体积电子显微镜，评价了间氯苯肼给药后ΔΨm丢失诱导转化过程中的早期结构变化。我们发现，大多数线粒体在CCCP暴露后10 分钟内由管状变为球状，没有融合或分裂，通常呈环状。相反，大多数环状线粒体没有真正的通孔；相反，它们有不同的凹痕，47%的线粒体呈气孔形状，有花瓶状的空洞，如果长管形状缩短为球状，这是最稳定的物理结构，没有任何结构支撑。我们的结果提示，ΔΨm的丢失引发了线粒体结构支持机制的崩溃。
Loss of mitochondrial membrane potential (ΔΨm) triggers dramatic structural changes in mitochondria from a tubular to globular shape, referred to as mitochondrial fragmentation; the resulting globular mitochondria are called swelled or ring/doughnut mitochondria. We evaluated the early period of structural changes during the ΔΨm loss-induced transformation after carbonyl cyanide m-chlorophenyl hydrazine (CCCP) administration using a newly developed correlative microscopic method combined with fluorescence microscopic live imaging and volume electron microscopy. We found that most mitochondria changed from a tubular shape to a globular shape without fusion or fission and typically showed ring shapes within 10 min after CCCP exposure. In contrast, most ring mitochondria did not have a true through hole; rather, they had various indents, and 47% showed stomatocyte shapes with vase-shaped cavities, which is the most stable physical structure without any structural support if the long tubular shape shortens into a sphere. Our results suggested that loss of ΔΨm triggered collapse of mitochondrial structural support mechanisms.
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影响因子：
2.4
作者：
Sharma J;Johnston MV;Hossain MA
通讯作者：
Hossain MA
影响因子：
12.4
作者：
通讯作者：
--
影响因子：
21.3
作者：
Bleazard, W;McCaffery, JM;Shaw, JM
通讯作者：
Shaw, JM
影响因子：
4.8
作者：
Ding, Wen-Xing;Li, Min;Yin, Xiao-Ming
通讯作者：
Yin, Xiao-Ming
DOI：
10.1016/s0006-291x(03)00050-0
发表时间：
2003-02-21
影响因子：
3.1
作者：
Ishihara, N;Jofuku, A;Mihara, K
通讯作者：
Mihara, K