Universal microbial diagnostics using random DNA probes.
Universal microbial diagnostics using random DNA probes.
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DOI：
10.1126/sciadv.1600025
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发表时间：
2016-09
期刊：
影响因子：
13.6
通讯作者：
Baraniuk RG
中科院分区：
文献类型：
--
作者：
Aghazadeh A;Lin AY;Sheikh MA;Chen AL;Atkins LM;Johnson CL;Petrosino JF;Drezek RA;Baraniuk RG
A new diagnostic platform based on randomized DNA probes can screen for common human pathogens. Early identification of pathogens is essential for limiting development of therapy-resistant pathogens and mitigating infectious disease outbreaks. Most bacterial detection schemes use target-specific probes to differentiate pathogen species, creating time and cost inefficiencies in identifying newly discovered organisms. We present a novel universal microbial diagnostics (UMD) platform to screen for microbial organisms in an infectious sample, using a small number of random DNA probes that are agnostic to the target DNA sequences. Our platform leverages the theory of sparse signal recovery (compressive sensing) to identify the composition of a microbial sample that potentially contains novel or mutant species. We validated the UMD platform in vitro using five random probes to recover 11 pathogenic bacteria. We further demonstrated in silico that UMD can be generalized to screen for common human pathogens in different taxonomy levels. UMD’s unorthodox sensing approach opens the door to more efficient and universal molecular diagnostics.
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影响因子：
3
作者：
Mohtashemi M;Walburger DK;Peterson MW;Sutton FN;Skaer HB;Diggans JC
通讯作者：
Diggans JC
影响因子：
38.3
作者：
通讯作者：
--
影响因子：
2.2
作者：
Pechorsky, Alina;Nitzan, Yeshayahu;Lazarovitch, Tsilia
通讯作者：
Lazarovitch, Tsilia
DOI：
10.1073/pnas.0502269102
发表时间：
2005-07-05
影响因子：
11.1
作者：
Donoho, DL;Tanner, J
通讯作者：
Tanner, J
DOI：
10.1155/2009/162824
发表时间：
2009
期刊：
EURASIP journal on bioinformatics & systems biology
影响因子：
--
作者：
Dai W;Sheikh MA;Milenkovic O;Baraniuk RG
通讯作者：
Baraniuk RG