Tumor cytolysis by lymphocytes infiltrating ovarian malignant ascites.
Tumor cytolysis by lymphocytes infiltrating ovarian malignant ascites.
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肿瘤细胞被淋巴细胞浸润而溶解卵巢恶性腹水。
作者：
Constantin G. Loannides;C. Platsoucas;S. Rashed;J. Wharton;C. Edwards;R. Freedman
从卵巢腺癌患者的腹水中分离出肿瘤相关淋巴细胞（TAL）。在中等浓度（50-200 cetus单位/ml）的重组白细胞介素2存在下，这些细胞作为CD3+ CD4+或CD3+ CD8+培养物增殖和扩增100-600倍，并达到高数量（5 x 10(8)-1 x 10(9)）。对来自 15 名患者的 16 份 TAL 样本进行扩增后，7 个分离的 T 细胞受体 (TCR) (alpha beta)+ CD3+ CD8+ CD4- 表型的卵巢细胞毒性 T 淋巴细胞系中的 5 个表现出针对自体肿瘤靶标的优先细胞溶解活性，并且针对同种异体肿瘤靶标和自然杀伤敏感细胞系 K562 的细胞溶解活性显着降低。 CD8+ TAL的细胞溶解活性被可操作的抗TCR（αβ）单克隆抗体和CD3分化抗原特异性单克隆抗体抑制，表明TCR和CD3参与细胞溶解过程。其他 TAL 培养物对自体和同种异体肿瘤表现出类似的细胞溶解活性。这些 TAL 的表型主要是 TCR (alpha beta)+ CD3+ CD4+ CD8-。从代表性 TAL 中分离的某些 CD3+ CD8+ T 细胞克隆表现出优先的自体肿瘤特异性细胞毒性，这可能受到主要组织相容性复合物的限制。其他 CD3+ CD8+ 和 CD3+ CD4+ 克隆表现出非主要组织相容性复合体限制的细胞毒性。这些结果表明，卵巢恶性腹水中存在的CD3+CD4+和CD3+CD8+T细胞可以在体外作为T细胞系大量且长时间地增殖。这一发现对于卵巢肿瘤特异性细胞毒性 T 淋巴细胞的进一步研究和未来的过继特异性免疫治疗研究可能具有重要意义。
Tumor-associated lymphocytes (TAL) were isolated from the ascitic fluid of patients with adenocarcinoma of the ovary. These cells proliferated and expanded by 100-600-fold as either CD3+ CD4+ or CD3+ CD8+ cultures in the presence of moderate concentrations (50-200 cetus units/ml) of recombinant interleukin 2 and reached high numbers (5 x 10(8)-1 x 10(9)). After expansion of 16 TAL samples from 15 patients, 5 of the 7 isolated ovarian cytotoxic T-lymphocyte cell lines of T-cell receptor (TCR) (alpha beta)+ CD3+ CD8+ CD4- phenotype exhibited preferential cytolytic activity against autologous tumor targets and significantly lower cytolytic activity against allogeneic tumor targets and the natural killer-sensitive cell line K562. The cytolytic activity of the CD8+ TAL was inhibited by operationally anti-TCR (alpha beta) monoclonal antibody and monoclonal antibody specific for the CD3 differentiation antigen, indicating that the TCR and CD3 are involved in the cytolytic process. The other TAL cultures demonstrated similar cytolytic activity against both autologous and allogeneic tumors. The phenotype of these TAL was predominantly TCR (alpha beta)+ CD3+ CD4+ CD8-. Certain CD3+ CD8+ T-cell clones isolated from representative TAL exhibited preferential autologous tumor-specific cytotoxicity that may be major histocompatibility complex restricted. Other CD3+ CD8+ and CD3+ CD4+ clones exhibited nonmajor histocompatibility complex restricted cytotoxicity. These results demonstrate that CD3+ CD4+ and CD3+ CD8+ T-cells present in the ovarian malignant ascites can be propagated in large numbers and for long time intervals as T-cell lines in vitro. This finding may be significant for further investigation of ovarian tumor-specific cytotoxic T-lymphocytes and future adoptive specific immunotherapy studies.
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卵巢肿瘤浸润淋巴细胞：表型和抗肿瘤活性。
DOI：
--
发表时间：
1988
期刊：
Natural immunity and cell growth regulation
影响因子：
--
作者：
Lotzova,E
通讯作者：
Lotzova,E
用双特异性单克隆抗体繁殖的 CD4 和 CD8 肿瘤浸润淋巴细胞的表征。
DOI：
--
发表时间：
1989
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Wong,JT;Pinto,CE;Gifford,JD;Kurnick,JT;Kradin,RL
通讯作者：
Kradin,RL
影响因子：
4.4
作者：
N. Chakraborty;D. Twardzik;M. Sivanandham;M. T. Ergin;K. Hellstrom;B. Mukherji
通讯作者：
N. Chakraborty;D. Twardzik;M. Sivanandham;M. T. Ergin;K. Hellstrom;B. Mukherji
通过抗肽 γ 链特异性单克隆抗体鉴定人和小鼠中的第二种 T 细胞抗原受体。
DOI：
10.1073/pnas.84.12.4244
发表时间：
1987
影响因子：
11.1
作者：
Ioannides,CG;Itoh,K;Fox,FE;Pahwa,R;Good,RA;Platsoucas,CD
通讯作者：
Platsoucas,CD
来自正常供体的 CD4-CD8-T 细胞克隆表达的 γ δ T 细胞抗原受体的分子异质性：二硫键连接和非二硫键连接的受体都是 δ TCS1。
DOI：
10.1073/pnas.86.7.2326
发表时间：
1989
影响因子：
11.1
作者：
Seki,H;Nanno,M;Chen,PF;Itoh,K;Ioannides,C;Good,RA;Platsoucas,CD
通讯作者：
Platsoucas,CD