Mechanical induction of dentin-like differentiation by adult mouse bone marrow stromal cells using compressive scaffolds.
Mechanical induction of dentin-like differentiation by adult mouse bone marrow stromal cells using compressive scaffolds.
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DOI：
10.1016/j.scr.2017.08.011
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发表时间：
2017-10
影响因子：
1.2
通讯作者：
Ingber DE
中科院分区：
文献类型：
--
作者：
Hashmi B;Mammoto T;Weaver J;Ferrante T;Jiang A;Jiang E;Feliz J;Ingber DE
Tooth formation during embryogenesis is controlled through a complex interplay between mechanical and chemical cues. We have previously shown that physical cell compaction of dental mesenchyme cells during mesenchymal condensation is responsible for triggering odontogenic differentiation during embryogenesis, and that expression of collagen VI stabilizes this induction. In addition, we have shown that synthetic polymer scaffolds that artificially induce cell compaction can induce embryonic mandible mesenchymal cells to initiate tooth differentiation both in vitro and in vivo. As embryonic cells would be difficult to use for regenerative medicine applications, here we explored whether compressive scaffolds coated with collagen VI can be used to induce adult bone marrow stromal cells (BMSCs) to undergo an odontogenic lineage switch. These studies revealed that when mouse BMSCs are compressed using these scaffolds they increase expression of critical markers of tooth differentiation in vitro, including the key transcription factors Pax9 and Msx1. Implantation under the kidney capsule of contracting scaffolds bearing these cells in mice also resulted in local mineralization, calcification and production of dentin-like tissue. These findings show that these chemically-primed compressive scaffolds can be used to induce adult BMSCs to undergo a lineage switch and begin to form dentin-like tissue, thus raising the possibility of using adult BMSCs for future tooth regeneration applications.
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影响因子：
29.4
作者：
Hashmi, Basma;Zarzar, Lauren D.;Mammoto, Tadanori;Mammoto, Akiko;Jiang, Amanda;Aizenberg, Joanna;Ingber, Donald E.
通讯作者：
Ingber, Donald E.
DOI：
10.1002/dvdy.24264
发表时间：
2015-06
期刊：
Developmental dynamics : an official publication of the American Association of Anatomists
影响因子：
--
作者：
Mammoto T;Mammoto A;Jiang A;Jiang E;Hashmi B;Ingber DE
通讯作者：
Ingber DE
DOI：
10.1073/pnas.1137104100
发表时间：
2003-05-27
影响因子：
11.1
作者：
Mitsiadis, TA;Chéraud, Y;Fontaine-Pérus, J
通讯作者：
Fontaine-Pérus, J
影响因子：
2.9
作者：
KARCHERDJURICIC, V;STAUBLI, A;RUCH, JV
通讯作者：
RUCH, JV
影响因子：
2.7
作者：
Rothova, Michaela;Peterkova, Renata;Tucker, Abigail S.
通讯作者：
Tucker, Abigail S.