Adipose tissue expression of CCL19 chemokine is positively associated with insulin resistance.
Adipose tissue expression of CCL19 chemokine is positively associated with insulin resistance.
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DOI：
10.1002/dmrr.3087
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发表时间：
2019-03
期刊：
影响因子：
--
通讯作者：
Ahmad R
中科院分区：
文献类型：
--
作者：
Kochumon S;Al-Rashed F;Abu-Farha M;Devarajan S;Tuomilehto J;Ahmad R
脂肪组织（AT）产生的趋化因子参与肥胖人类和啮齿动物慢性低度炎症的发展。人们对肥胖症中AT CCL 19的表达及其与代谢性炎症和胰岛素抵抗的关系知之甚少。本研究旨在探讨CCL 19基因表达对皮下AT和胰岛素抵抗中炎症标志物的影响。从56名非糖尿病患者（26名肥胖、21名超重和9名消瘦）中采集皮下脂肪样本。使用真实的实时RT-PCR测定CCL 19和炎症标志物的表达。通过ELISA测定血浆C反应蛋白（CRP）和脂联素。采用稳态模型评价指数（HOMA）评价胰岛素敏感性。肥胖组CCL 19表达显著高于消瘦组（P < 0.034）。CCL 19的表达与体重指数呈正相关（r = 0.253; P = 0.049）。CCL 19表达与IL-8正相关（r = 0.39; P = 0.006），IL-12（r = 0.43; P = 0.003）、IP-10（r = 0.25; P = 0.07）、CCL5（r = 0.37; P = 0.011）、CCR2（r = 0.44; P = 0.001）和CCR5（r = 0.35; P = 0.009）。此外，CCL 19与甘油三酯呈正相关，（TG：r = 0.41; P = 0.001），空腹血糖（FBG：r = 0.49; P < 0.0001），糖化血红蛋白（HbA1c：r = 0.396; P = 0.001）和CRP（r = 0.387; P = 0.019），而与HDL胆固醇（r =-0.282; P = 0.035）和脂联素（-0.393; P = 0.019）呈负相关。值得注意的是，HOMA-IR与CCL 19呈正相关（r = 0.38; P = 0.01）。在多元回归分析中，CCL 19是IL-8和IL-12的独立预测因子。这些数据表明，肥胖症中CCL 19的AT表达增加可能代表代谢性炎症和胰岛素抵抗之间的分子联系。
Chemokines produced by adipose tissue (AT) are involved in the development of chronic low‐grade inflammation in obese humans and rodents. AT CCL19 expression in obesity and its association with metabolic inflammation and insulin resistance are poorly understood. This study aimed to investigate the effects of CCL19 gene expression on inflammatory markers in subcutaneous AT and insulin resistance. Subcutaneous adipose samples were collected from 56 non‐diabetic (26—obese, 21—overweight, and 9—lean) individuals. Expression of CCL19 and inflammatory markers was determined using real‐time RT‐PCR. Plasma C‐reactive protein (CRP) and adiponectin were measured by ELISA. Insulin sensitivity was assessed using homeostasis model assessment index (HOMA). CCL19 expression was significantly higher in obese compared with lean individuals (P < 0.034). The elevated expression of CCL19 associated positively with body mass index (r = 0.253; P = 0.049). CCL19 expression correlated positively with IL‐8 (r = 0.39; P = 0.006), IL‐12 (r = 0.43; P = 0.003), IP‐10 (r = 0.25; P = 0.07), CCL5 (r = 0.37; P = 0.011), CCR2 (r = 0.44; P = 0.001), and CCR5 (r = 0.35; P = 0.009). Additionally, CCL19 was positively correlated with triglycerides (TG: r = 0.41; P = 0.001), fasting blood glucose (FBG: r = 0.49; P < 0.0001), glycated haemoglobin (HbA1c: r = 0.396; P = 0.001), and CRP (r = 0.387; P = 0.019) whereas it had negative association with HDL cholesterol (r = −0.282; P = 0.035) and adiponectin (−0.393; P = 0.019). Notably, HOMA‐IR correlated positively with CCL19 (r = 0.38; P = 0.01). In multiple regression analysis, CCL19 is an independent predictor of IL‐8 and IL‐12. These data demonstrate that increased AT expression of CCL19 in obesity may represent a molecular link between metabolic inflammation and insulin resistance.
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DOI：
10.1186/1476-9255-9-48
发表时间：
2012-11-28
期刊：
Journal of inflammation (London, England)
影响因子：
--
作者：
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DOI：
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发表时间：
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DOI：
10.1152/ajpendo.00269.2003
发表时间：
2004-01-01
影响因子：
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