Shanghai Neisseria gonorrhoeae Isolates Exhibit Resistance to Extended-Spectrum Cephalosporins and Clonal Distribution.
Shanghai Neisseria gonorrhoeae Isolates Exhibit Resistance to Extended-Spectrum Cephalosporins and Clonal Distribution.
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DOI：
10.3389/fmicb.2020.580399
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发表时间：
2020
影响因子：
5.2
通讯作者：
Gu W
中科院分区：
文献类型：
--
作者：
Dong Y;Yang Y;Wang Y;Martin I;Demczuk W;Gu W
对超广谱头孢菌素（ESCsR）具有耐药性（R）的淋病奈瑟菌菌株的出现代表了无法治疗的淋球菌感染对公共卫生的威胁。本研究旨在探讨上海地区老年人ESCR的患病率及其分子机制。淋病分离株。共366个。淋病分离株于2017年在上海收集。采用琼脂稀释法测定对头孢曲松（CRO）、头孢克肟（CFM）、阿奇霉素（AZM）、环丙沙星（CIP）、大观霉素、青霉素和四环素的敏感性。对124个分离株的子集进行了9个抗菌素耐药相关基因的系统发育分析，佩纳、porB、ponA、mtrR、23 S rRNA、gyrA、parC、16 S rRNA和rpsE。约20.0%的菌株表现出CFMR [最小抑菌浓度（MIC）>0.125 mg/L]，5.5%为CROR（MIC > 0.125 mg/L）。总的来说，72.7%的ESCR分离株是克隆的，并与嵌合佩纳10和60等位基因相关。在非克隆性ESCR中观察到非嵌合佩纳18等位基因和佩纳A501 T、G542 S和PorB 1b G213 S/Y的替换。约6.8%的分离株显示AZM MIC高于流行病学临界值（ECOFF，1 mg/L），与23 S rRNA A2059 G突变相关，未显示克隆分布。几乎所有的分离株都是CIPR（对环丙沙星的耐药性），并与GyrA-91/92和ParC-85/86/87/88/89/91变异相关。将具有ParC S88 P取代的分离物聚类到ESCR进化枝中。上海分离株ESCR水平高，抗性模式明显。
The emergence of Neisseria gonorrhoeae strains with resistance (R) to extended-spectrum cephalosporins (ESCsR) represents a public health threat of untreatable gonococcal infections. This study was designed to determine the prevalence and molecular mechanisms of ESCR of Shanghai N. gonorrhoeae isolates. A total of 366 N. gonorrhoeae isolates were collected in 2017 in Shanghai. Susceptibility to ceftriaxone (CRO), cefixime (CFM), azithromycin (AZM), ciprofloxacin (CIP), spectinomycin, penicillin, and tetracycline was determined using the agar dilution method. A subset of 124 isolates was subjected to phylogenetic analysis for nine antimicrobial resistance-associated genes, i.e., penA, porB, ponA, mtrR, 23S rRNA, gyrA, parC, 16S rRNA, and rpsE. Approximately 20.0% of the isolates exhibited CFMR [minimum inhibitory concentration (MIC) >0.125 mg/L], and 5.5% were CROR (MIC > 0.125 mg/L). In total, 72.7% of ESCR isolates were clonal and associated with mosaic penA 10 and 60 alleles. Non-mosaic penA 18 allele and substitutions of PenA A501T, G542S, and PorB1b G213S/Y were observed in non-clonal ESCR. Approximately 6.8% of the isolates showed AZM MIC above the epidemiological cutoff (ECOFF, 1 mg/L), were associated with 23S rRNA A2059G mutation, and did not exhibit clonal distribution. Almost all isolates were CIPR (resistance to ciprofloxacin) and associated with GyrA-91/92 and ParC-85/86/87/88/89/91 alterations. Isolates with ParC S88P substitution were clustered into the ESCR clade. The Shanghai isolates exhibited a high level of ESCR and distinct resistant patterns.
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影响因子：
3.2
作者：
Barry PM;Klausner JD
通讯作者：
Klausner JD
影响因子：
11.8
作者：
Lahra MM;Martin I;Demczuk W;Jennison AV;Lee KI;Nakayama SI;Lefebvre B;Longtin J;Ward A;Mulvey MR;Wi T;Ohnishi M;Whiley D
通讯作者：
Whiley D
DOI：
10.1093/bioinformatics/btx610
发表时间：
2018-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Hadfield J;Croucher NJ;Goater RJ;Abudahab K;Aanensen DM;Harris SR
通讯作者：
Harris SR
影响因子：
56.3
作者：
Grad, Yonatan H.;Kirkcaldy, Robert D.;Trees, David;Dordel, Janina;Harris, Simon R.;Goldstein, Edward;Weinstock, Hillard;Parkhill, Julian;Hanage, William P.;Bentley, Stephen;Lipsitch, Marc
通讯作者：
Lipsitch, Marc
影响因子：
5.2
作者：
Lee, Sang-Guk;Lee, Hyukmin;Lee, Kyungwon
通讯作者：
Lee, Kyungwon