Dorsal horn-enriched genes identified by DNA microarray, in situ hybridization and immunohistochemistry.
Dorsal horn-enriched genes identified by DNA microarray, in situ hybridization and immunohistochemistry.
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DOI：
10.1186/1471-2202-3-11
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发表时间：
2002-08-20
期刊：
影响因子：
2.4
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Sun H;Xu J;Della Penna KB;Benz RJ;Kinose F;Holder DJ;Koblan KS;Gerhold DL;Wang H
脊髓背侧神经元在伤害感受和疼痛中起重要作用。这些神经元接收来自外周感觉神经元的输入，然后将信号传输到大脑，以及接收和整合来自大脑的下行控制信号。许多对疼痛传递重要的分子已被证明定位于脊髓的背角。进一步了解背角神经元的分子相互作用和信号通路将需要更好地了解脊髓背角的分子神经解剖学。利用基因芯片和实时荧光定量PCR技术对脊髓背角富集表达的基因进行大规模筛选。除了已知在脊髓背侧特异性表达的基因外，还发现其他神经肽、受体、离子通道和信号分子在脊髓背侧富集。原位杂交和免疫组织化学揭示了这些基因的一个子集的细胞表达。还在脊神经结扎（SNL）神经性疼痛模型中研究了基因子集的调节。总之，我们发现在脊髓背侧富集的基因不在神经性疼痛的脊神经结扎模型中被发现上调的基因之列。这项研究还提供了一个水平的验证使用DNA微阵列结合我们的新的分析算法（SAFER）的基因表达的差异进行鉴定。这项研究确定了脊髓背角中富集的分子，并提供了脊髓中的分子神经解剖学，这将有助于理解伤害感受和疼痛中重要的分子机制。
Neurons in the dorsal spinal cord play important roles in nociception and pain. These neurons receive input from peripheral sensory neurons and then transmit the signals to the brain, as well as receive and integrate descending control signals from the brain. Many molecules important for pain transmission have been demonstrated to be localized to the dorsal horn of the spinal cord. Further understanding of the molecular interactions and signaling pathways in the dorsal horn neurons will require a better knowledge of the molecular neuroanatomy in the dorsal spinal cord. A large scale screening was conducted for genes with enriched expression in the dorsal spinal cord using DNA microarray and quantitative real-time PCR. In addition to genes known to be specifically expressed in the dorsal spinal cord, other neuropeptides, receptors, ion channels, and signaling molecules were also found enriched in the dorsal spinal cord. In situ hybridization and immunohistochemistry revealed the cellular expression of a subset of these genes. The regulation of a subset of the genes was also studied in the spinal nerve ligation (SNL) neuropathic pain model. In general, we found that the genes that are enriched in the dorsal spinal cord were not among those found to be up-regulated in the spinal nerve ligation model of neuropathic pain. This study also provides a level of validation of the use of DNA microarrays in conjunction with our novel analysis algorithm (SAFER) for the identification of differences in gene expression. This study identified molecules that are enriched in the dorsal horn of the spinal cord and provided a molecular neuroanatomy in the spinal cord, which will aid in the understanding of the molecular mechanisms important in nociception and pain.
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DOI：
10.1073/pnas.011404098
发表时间：
2001-01-02
影响因子：
11.1
作者：
Li, C;Wong, WH
通讯作者：
Wong, WH
影响因子：
3.3
作者：
Lai, CC;Wu, SY;Dun, NJ
通讯作者：
Dun, NJ
影响因子：
3
作者：
MERCHENTHALER, I;MADERDRUT, JL;CONLON, JM
通讯作者：
CONLON, JM
影响因子：
3.3
作者：
MARKSTEINER, J;KIRCHMAIR, R;WINKLER, H
通讯作者：
WINKLER, H
影响因子：
3.3
作者：
KIYAMA, H;EMSON, PC
通讯作者：
EMSON, PC