Brillouin-Raman microspectroscopy for the morpho-mechanical imaging of human lamellar bone.
Brillouin-Raman microspectroscopy for the morpho-mechanical imaging of human lamellar bone.
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DOI：
10.1098/rsif.2021.0642
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发表时间：
2022-03
期刊：
影响因子：
--
通讯作者：
Fioretto D
中科院分区：
文献类型：
--
作者：
Alunni Cardinali M;Di Michele A;Mattarelli M;Caponi S;Govoni M;Dallari D;Brogini S;Masia F;Borri P;Langbein W;Palombo F;Morresi A;Fioretto D
骨具有复杂的结构，其特征在于从亚微米级开始的分层组织。因此，在这个尺度上分析骨的机械和结构特性对于理解其生理学、物理特性和化学成分之间的关系至关重要。在这里，我们揭示了布里渊-拉曼显微光谱（BRaMS）的潜力，这是一种新兴的相关光学方法，可以同时评估骨力学和化学与微米分辨率。相关的高光谱成像，在人体骨干环上进行，揭示了一个复杂的微结构，反映在极其丰富和信息丰富的光谱。提出了一种创新的力学性能分析方法，映射软硬组织区域的混合，并揭示了重塑过程，营养物质运输和结构支持所涉及的区域的共存。矿化区域出现弹性不均匀，类似于骨单位的层状模式，而通过扫描电子显微镜的拉曼和能量色散X射线图像显示矿物含量的总体均匀分布，表明其他结构因素是层状微观力学异质性的原因。这些结果，除了提供一个重要的洞察皮质骨组织的性质，突出的潜力BRaMS访问的起源各向异性的机械性能，这是几乎无处不在的其他生物组织。
Bone has a sophisticated architecture characterized by a hierarchical organization, starting at the sub-micrometre level. Thus, the analysis of the mechanical and structural properties of bone at this scale is essential to understand the relationship between its physiology, physical properties and chemical composition. Here, we unveil the potential of Brillouin–Raman microspectroscopy (BRaMS), an emerging correlative optical approach that can simultaneously assess bone mechanics and chemistry with micrometric resolution. Correlative hyperspectral imaging, performed on a human diaphyseal ring, reveals a complex microarchitecture that is reflected in extremely rich and informative spectra. An innovative method for mechanical properties analysis is proposed, mapping the intermixing of soft and hard tissue areas and revealing the coexistence of regions involved in remodelling processes, nutrient transportation and structural support. The mineralized regions appear elastically inhomogeneous, resembling the pattern of the osteons' lamellae, while Raman and energy-dispersive X-ray images through scanning electron microscopy show an overall uniform distribution of the mineral content, suggesting that other structural factors are responsible for lamellar micromechanical heterogeneity. These results, besides giving an important insight into cortical bone tissue properties, highlight the potential of BRaMS to access the origin of anisotropic mechanical properties, which are almost ubiquitous in other biological tissues.
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影响因子：
5.8
作者：
Donnadio, Anna;Bini, Marzia;Nocchetti, Morena
通讯作者：
Nocchetti, Morena
影响因子：
13.6
作者：
Bailey M;Alunni-Cardinali M;Correa N;Caponi S;Holsgrove T;Barr H;Stone N;Winlove CP;Fioretto D;Palombo F
通讯作者：
Palombo F
影响因子：
19.4
作者：
Mattana, Sara;Mattarelli, Maurizio;Caponi, Silvia
通讯作者：
Caponi, Silvia
影响因子：
2.8
作者：
Meng, Zhaokai;Lopez, Sandra C. Bustamante;Yakovlev, Vladislav V.
通讯作者：
Yakovlev, Vladislav V.
使用高光谱相干抗螺旋体拉曼散射显微镜的定量化学成像和无监督分析。
影响因子：
7.4
作者：
Masia, Francesco;Glen, Adam;Stephens, Phil;Borri, Paola;Langbein, Wolfgang
通讯作者：
Langbein, Wolfgang