Controllable mineral coatings on PCL scaffolds as carriers for growth factor release.
Controllable mineral coatings on PCL scaffolds as carriers for growth factor release.
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DOI：
10.1016/j.biomaterials.2011.09.095
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发表时间：
2012-01
期刊：
影响因子：
14
通讯作者：
Murphy, William L.
中科院分区：
文献类型：
--
作者：
Suarez-Gonzalez, Darilis;Barnhart, Kara;Migneco, Francesco;Flanagan, Colleen;Hollister, Scott J.;Murphy, William L.
关键词：
在这项研究中，我们在聚己内酯支架上开发了矿物涂层，作为生长因子结合和释放的模板。矿物涂层是使用仿生方法形成的，该方法包括在改良的模拟体液（mSBF）中孵育支架。为了调节矿物涂层的特性（我们假设这将决定生长因子的释放），我们在 mSBF 中使用了浓度为 4.2 mM、25 mM 和 100 mM 的碳酸盐 (HCO3)。使用扫描电子显微镜对形成的矿物涂层进行分析表明，具有不同形态的连续矿物层的生长。 X 射线衍射分析显示，在所有 HCO3 浓度下形成的涂层中，都有与羟基磷灰石相关的峰，羟基磷灰石是人体骨组织的主要无机成分。 HCO3 取代度增加的矿物涂层在缺乏钙和磷酸盐的环境中表现出更快的溶解动力学，但在具有上述离子的环境中表现出再沉淀。矿物涂层提供了生长因子结合和释放的有效机制。血管内皮生长因子 (VEGF) 和骨形态发生蛋白 2 (BMP2) 的肽版本与矿物涂层 PCL 支架的结合效率高达 90%。我们还证明了所有生长因子的持续释放，其释放动力学强烈依赖于矿物涂层的溶解度。
In this study, we have developed mineral coatings on polycaprolactone scaffolds to serve as templates for growth factor binding and release. Mineral coatings were formed using a biomimetic approach that consisted in the incubation of scaffolds in modified simulated body fluids (mSBF). To modulate the properties of the mineral coating, which we hypothesized would dictate growth factor release, we used carbonate (HCO3) concentration in mSBF of 4.2 mM, 25mM, and 100mM. Analysis of the mineral coatings formed using scanning electron microscopy indicated growth of a continuous layer of mineral with different morphologies. X-ray diffraction analysis showed peaks associated with hydroxyapatite, the major inorganic constituent of human bone tissue in coatings formed in all HCO3 concentrations. Mineral coatings with increased HCO3 substitution showed more rapid dissolution kinetics in an environment deficient in calcium and phosphate but showed re-precipitation in an environment with the aforementioned ions. The mineral coating provided an effective mechanism for growth factor binding and release. Peptide versions of vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP2) were bound with efficiencies up to 90% to mineral mineral-coated PCL scaffolds. We also demonstrated sustained release of all growth factors with release kinetics that were strongly dependent in the solubility of the mineral coating.
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影响因子：
9.7
作者：
Lee, Jae Sam;Lee, Jae Sung;Murphy, William L.
通讯作者：
Murphy, William L.
影响因子：
9.7
作者：
Choi, Siyoung;Murphy, William L.
通讯作者：
Murphy, William L.
DOI：
10.1073/pnas.0505047102
发表时间：
2005-10-04
影响因子：
11.1
作者：
D'Andrea, LD;Iaccarino, G;Pedone, C
通讯作者：
Pedone, C
影响因子：
3.8
作者：
Fu, Yin-Chih;Nie, Hemin;Wang, Chi-Hwa
通讯作者：
Wang, Chi-Hwa
影响因子：
4.3
作者：
Cochran, DI;Jones, AA;Howell, H
通讯作者：
Howell, H