Sustained plasmid DNA release from dissolving mineral coatings.
Sustained plasmid DNA release from dissolving mineral coatings.
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DOI：
10.1016/j.actbio.2010.03.020
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发表时间：
2010-09
影响因子：
9.7
通讯作者：
Murphy, William L.
中科院分区：
文献类型：
--
作者：
Choi, Siyoung;Murphy, William L.
磷酸钙 (Ca-P) 矿物质（例如羟基磷灰石）由于其晶体结构中存在阴离子和阳离子，能够结合多种生物分子。 Ca-P 矿物质结合和释放质粒 DNA 的良好特性，加上在常见生物材料表面形成可生物降解的 Ca-P 涂层的能力，为从各种生物材料中控制释放质粒 DNA 提供了潜在机制。在本研究中，我们假设聚丙交酯乙交酯 (PLG) 基质上形成的 Ca-P 涂层中质粒 DNA 的释放将取决于 Ca-P 矿物涂层的固有特性和周围的溶液条件。实验设计考虑两个一般参数：i）各种Ca-P矿物涂层在与生理条件相关的溶液环境中的稳定性； ii) 矿物质稳定性与持续质粒 DNA 释放之间的关系。我们的结果证实了先前的研究，这些研究证明了内在矿物成分与矿物稳定性之间的直接关系。此外，我们进一步证明周围溶液环境的离子组成和 pH 值可以显着影响矿物稳定性。反过来，矿物稳定性显着影响体外矿物涂层中质粒 DNA 的释放，并且可以通过控制不同溶液环境下的矿物特性来调节 DNA 释放效率。这些 Ca-P 矿物涂层可能是使用各种生物材料平台的质粒 DNA 递送应用的有用平台。
Calcium phosphate (Ca-P) minerals such as hydroxyapatite are able to bind a diverse range of biological molecules due to the presence of anions and cations in their crystal structure. The well-characterized ability of Ca-P minerals to bind and release plasmid DNA, coupled with the ability of biodegradable Ca-P coatings to form on the surface of common biomaterials, provides a potential mechanism for controlled release of plasmid DNA from various biomaterials. In this study we hypothesized that the release of plasmid DNA from Ca-P coatings formed on poly(lactide-co-glycolide) (PLG) substrates would be dependent on both the intrinsic properties of the Ca-P mineral coating and the surrounding solution conditions. Experiments were designed to consider two general parameters: i) the stability of various Ca-P mineral coatings in solution environments that are relevant to physiological conditions; and ii) the relationship between mineral stability and sustained plasmid DNA release. Our results corroborate previous studies that have demonstrated a direct relationship between intrinsic mineral composition and mineral stability. In addition, we further demonstrate that ion composition and pH of the surrounding solution environment can significantly influence mineral stability. In turn, mineral stability significantly influenced release of plasmid DNA from mineral coatings in vitro, and the DNA release efficiency could be tuned by controlling the mineral properties under various solution environments. These Ca-P mineral coatings may be a useful platform for plasmid DNA delivery applications using various biomaterial platforms.
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DOI：
10.1023/a:1011271713758
发表时间：
2001-01-01
影响因子：
3.7
作者：
Barrère, F;Layrolle, P;de Groot, K
通讯作者：
de Groot, K
影响因子：
4.9
作者：
Kang, Sun-Woong;Yang, Hee Seok;Kim, Byung-Soo
通讯作者：
Kim, Byung-Soo
DOI：
10.1023/a:1011174905511
发表时间：
2000-01-01
期刊：
BIOSEPARATION
影响因子：
--
作者：
Giovannini, R;Freitag, R
通讯作者：
Freitag, R
影响因子：
1.8
作者：
Koutsopoulos, S;Dalas, E
通讯作者：
Dalas, E
影响因子：
14.9
作者：
Jordan, M;Schallhorn, A;Wurm, FM
通讯作者：
Wurm, FM