Effect of tissue thickness and liquid composition on the penetration of long-lifetime reactive oxygen and nitrogen species (RONS) generated by a plasma jet
Effect of tissue thickness and liquid composition on the penetration of long-lifetime reactive oxygen and nitrogen species (RONS) generated by a plasma jet
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组织厚度和液体成分对等离子射流产生的长寿命活性氧和氮 (RONS) 渗透的影响
DOI：
10.1088/1361-6463/aad427
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发表时间：
2018-08
期刊：
影响因子：
--
通讯作者：
Lu X
中科院分区：
文献类型：
--
作者：
Nie L.;Yang Y.;Duan J.;Lu X.;Sun F.;He G.;Lu X.;Lu X;He G;Lu X
血浆医学的两个关键问题是血浆产生的活性氧和活性氮 (RONS) 能够渗透到组织的深度以及液体（细胞外液和细胞内液）成分如何影响 RONS 的浓度。本文以不同厚度的猪肌肉组织作为组织模式，研究组织厚度对RONS透过组织的影响。在组织下的接收室中使用六种不同类型的液体（无机组：双蒸水（DDW）、1%磷酸盐缓冲盐水、0.9% NaCl；有机组：5%葡萄糖、2%血清和10%血清溶液），以尝试了解液体成分对血浆产生的RONS（H2O2和）渗透的影响。研究发现，当使用厚度为 500 µm 的组织时，有机液体中的 H2O2 浓度比 DDW 中的浓度高约 20-30 倍。血清液体中的 和 浓度远高于所有其他液体，这可能是由于血浆与氨基酸和蛋白质发生反应。此外，有机溶液中的浓度高于相同实验条件下的浓度。此外，当血清百分比从2%增加到10%时，浓度急剧增加，但浓度显着下降。对于厚度为 500 µm 的组织尤其如此。一项新发现是，RONS 不仅通过扩散渗透组织，血浆和液体之间还发生反应，影响最终的 RONS 浓度。
Two of the key questions in plasma medicine are how deep the reactive oxygen and nitrogen species (RONS) generated by a plasma can penetrate into tissue and how the liquid (extracellular and intracellular fluid) composition affects the concentration of RONS. In this paper, different thicknesses of pig muscle tissue are used as a tissue mode to investigate the effect of tissue thickness on the penetration of RONS through tissue. Six different types of liquid (inorganic group: double-distilled water (DDW), 1% phosphate-buffered saline, 0.9% NaCl; organic group: 5% glucose, 2% serum and 10% serum solution) are used in the receiving chamber under the tissue in order to try to understand the effect of liquid composition on the penetration of RONS (H2O2, and ) generated by the plasma. It is found that when a tissue thickness of 500 µm is used the H2O2 concentrations in organic liquids are about 20–30 times higher than in DDW. The and concentrations in serum liquid are much higher than in all other liquids, which might be due to the plasma reacting with amino acids and proteins. Besides, the concentration in organic solution is higher than the concentration for the same experimental conditions. Furthermore, when the serum percentage is increased from 2% to 10%, the concentration increases dramatically but the concentration decreases significantly. This is especially true for a tissue thickness of 500 µm. One novel discovery is the RONS do not only penetrate the tissue by diffusion—there are also reactions between the plasma and the liquid which affect the final RONS concentration.
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影响因子：
3.4
作者：
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通讯作者：
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作者：
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影响因子：
4.6
作者：
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通讯作者：
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影响因子：
4.6
作者：
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通讯作者：
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