Regulating cell fate of human amnion epithelial cells using natural compounds: an example of enhanced neural and pigment differentiation by 3,4,5-tri-O-caffeoylquinic acid.
Regulating cell fate of human amnion epithelial cells using natural compounds: an example of enhanced neural and pigment differentiation by 3,4,5-tri-O-caffeoylquinic acid.
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DOI：
10.1186/s12964-020-00697-5
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发表时间：
2021-02-24
期刊：
影响因子：
--
通讯作者：
Isoda H
中科院分区：
文献类型：
--
作者：
Bejaoui M;Ferdousi F;Zheng YW;Oda T;Isoda H
在过去的几年里，人羊膜上皮细胞（hAEC），胎盘干细胞，越来越受到科学界的关注，因为它们显示出比其他类型的干细胞的几个优势，包括可用性，容易获得，排斥率降低，非致瘤性，和最小的法律的限制。最近，天然化合物被用于刺激干细胞分化和增殖，并增强其治疗疾病的潜力。此前曾报道多酚化合物3，4，5-三-O-咖啡酰奎宁酸（TCQA）可诱导人类神经干细胞分化，也可能影响黑素细胞干细胞分化。在这项研究中，TCQA在处理7天后在3D培养的hAEC上进行测试，然后在第0天和第7天对TCQA处理的和未处理的对照细胞进行微阵列基因表达谱分析。分析表明，TCQA处理显着丰富的色素和神经细胞集;此外，与神经发生，氧化还原过程，表皮发育和代谢相关的基因正调控。有趣的是，TCQA刺激细胞周期阻滞相关的途径和分化信号。另一方面，TCQA降低白细胞介素和细胞因子的表达，这是由于其作为多酚化合物的抗炎特性。结果被验证以突出TCQA对hAEC的主要活性，包括分化、细胞周期阻滞和抗炎。这项研究强调了hAEC在再生医学中的重要作用，以及使用天然化合物来调节它们的命运。视频摘要
Over the past years, Human Amnion Epithelial Cells (hAECs), a placental stem cell, are gaining higher attention from the scientific community as they showed several advantages over other types of stem cells, including availability, easy accessibility, reduced rejection rate, non-tumorigenicity, and minimal legal constraint. Recently, natural compounds are used to stimulate stem cell differentiation and proliferation and to enhance their disease-treating potential. A polyphenolic compound 3,4,5-Tri-O-Caffeoylquinic Acid (TCQA) has been previously reported to induce human neural stem cell differentiation and may affect melanocyte stem cell differentiation as well. In this study, TCQA was tested on 3D cultured hAECs after seven days of treatment, and then, microarray gene expression profiling was conducted of TCQA-treated and untreated control cells on day 0 and day 7. Analyses revealed that TCQA treatment significantly enriched pigment and neural cells sets; besides, genes linked with neurogenesis, oxidation–reduction process, epidermal development, and metabolism were positively regulated. Interestingly, TCQA stimulated cell cycle arrest-related pathways and differentiation signaling. On the other hand, TCQA decreased interleukins and cytokines expression and this due to its anti-inflammatory properties as a polyphenolic compound. Results were validated to highlight the main activities of TCQA on hAECs, including differentiation, cell cycle arrest, and anti-inflammatory. This study highlights the important role of hAECs in regenerative medicine and the use of natural compounds to regulate their fate. Video abstract
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影响因子：
2.4
作者：
Beck HN;Drahushuk K;Jacoby DB;Higgins D;Lein PJ
通讯作者：
Lein PJ
影响因子：
2.7
作者：
Cohen, Malkiel A.;Itsykson, Pavel;Reubinoff, Benjamin E.
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Reubinoff, Benjamin E.
影响因子：
3.1
作者：
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影响因子：
5.1
作者：
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通讯作者：
Perricaudet, M.
影响因子：
5.5
作者：
Bejaoui, Meriem;Villareal, Myra O.;Isoda, Hiroko
通讯作者：
Isoda, Hiroko