Bone morphogenetic protein-5 (BMP-5) promotes dendritic growth in cultured sympathetic neurons.
Bone morphogenetic protein-5 (BMP-5) promotes dendritic growth in cultured sympathetic neurons.
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DOI：
10.1186/1471-2202-2-12
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发表时间：
2001
期刊：
影响因子：
2.4
通讯作者：
Lein PJ
中科院分区：
文献类型：
--
作者：
Beck HN;Drahushuk K;Jacoby DB;Higgins D;Lein PJ
BMP-5在整个发育和成年期的神经系统中表达。然而，其对神经组织的影响尚未明确。BMP-5是BMP的60 A亚组的成员，其其他成员已显示刺激中枢和外周神经元中的树突生长。因此，我们研究了BMP-5类似地增强培养的交感神经元中树突生长的可能性。在没有血清或神经胶质细胞的情况下培养的交感神经元不形成树突;然而，添加BMP-5会导致这些神经元延长多个树突状突起，这之前会增加Smad-1转录因子的磷酸化。BMP-5的树突促进活性被BMP拮抗剂头蛋白和卵泡抑素以及BMPR-IA-Fc嵌合蛋白显著抑制。RT-PCR和免疫细胞化学分析表明，BMP-5的mRNA和蛋白质的表达在上级颈神经节（SCG）的树突状乔木的初始生长和快速扩张的时间。这些数据表明BMP-5在调节交感神经元中的树突生长中的作用。介导BMP-5的树突促进活性的信号传导途径可能涉及与BMPR-IA的结合和Smad-1的活化，并且BMP拮抗剂如头蛋白和卵泡抑素的相对水平可以调节BMP-5信号传导。由于BMP-5不仅在发育中而且在成人神经系统中以相对高的水平表达，这些发现表明BMP-5不仅在发育中而且在成人神经系统中调节树突形态的可能性。
BMP-5 is expressed in the nervous system throughout development and into adulthood. However its effects on neural tissues are not well defined. BMP-5 is a member of the 60A subgroup of BMPs, other members of which have been shown to stimulate dendritic growth in central and peripheral neurons. We therefore examined the possibility that BMP-5 similarly enhances dendritic growth in cultured sympathetic neurons. Sympathetic neurons cultured in the absence of serum or glial cells do not form dendrites; however, addition of BMP-5 causes these neurons to extend multiple dendritic processes, which is preceded by an increase in phosphorylation of the Smad-1 transcription factor. The dendrite-promoting activity of BMP-5 is significantly inhibited by the BMP antagonists noggin and follistatin and by a BMPR-IA-Fc chimeric protein. RT-PCR and immunocytochemical analyses indicate that BMP-5 mRNA and protein are expressed in the superior cervical ganglia (SCG) during times of initial growth and rapid expansion of the dendritic arbor. These data suggest a role for BMP-5 in regulating dendritic growth in sympathetic neurons. The signaling pathway that mediates the dendrite-promoting activity of BMP-5 may involve binding to BMPR-IA and activation of Smad-1, and relative levels of BMP antagonists such as noggin and follistatin may modulate BMP-5 signaling. Since BMP-5 is expressed at relatively high levels not only in the developing but also the adult nervous system, these findings suggest the possibility that BMP-5 regulates dendritic morphology not only in the developing, but also the adult nervous system.
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影响因子：
5.3
作者：
Le Roux, P;Behar, S;Charette, M
通讯作者：
Charette, M
DOI：
10.1073/pnas.96.5.2439
发表时间：
1999-03-02
影响因子：
11.1
作者：
Golden, JA;Bracilovic, A;Grinspan, JB
通讯作者：
Grinspan, JB
影响因子：
3.3
作者：
Farkas, LM;Jászai, J;Krieglstein, K
通讯作者：
Krieglstein, K
DOI：
10.1016/0736-5748(96)00008-1
发表时间：
1996-06-01
影响因子：
1.8
作者：
Lein, P;Guo, X;Higgins, D
通讯作者：
Higgins, D
影响因子：
16.2
作者：
Lo, LC;Morin, X;Anderson, DJ
通讯作者：
Anderson, DJ