Combined single-cell and spatial transcriptomics reveal the molecular, cellular and spatial bone marrow niche organization.
Combined single-cell and spatial transcriptomics reveal the molecular, cellular and spatial bone marrow niche organization.
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DOI：
10.1038/s41556-019-0439-6
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发表时间：
2020-01
影响因子：
21.3
通讯作者：
Haas S
中科院分区：
文献类型：
--
作者：
Baccin C;Al-Sabah J;Velten L;Helbling PM;Grünschläger F;Hernández-Malmierca P;Nombela-Arrieta C;Steinmetz LM;Trumpp A;Haas S
The bone marrow (BM) constitutes the primary site for life-long blood production and skeletal regeneration. However, its cellular composition and the spatial organization into distinct ‘niches’ remains controversial. Here, we combine single-cell and spatially resolved transcriptomics to systematically map the molecular and cellular composition of the endosteal, sinusoidal, and arteriolar BM niches. This allowed us to transcriptionally profile all major BM resident cell types, determine their localization, and clarify the cellular and spatial sources of key growth factors and cytokines. Our data demonstrate that previously unrecognized Cxcl12-abundant reticular (CAR) cell subsets (i.e. Adipo- and Osteo- CAR cells) differentially localize to sinusoidal or arteriolar surfaces, locally act as ‘professional cytokine secreting cells’, and thereby establish distinct peri-vascular micro-niches. Importantly, we also demonstrate that the 3-dimensional organization of the BM can be accurately inferred from single-cell gene expression data using the newly developed RNA-Magnet algorithm. Together, our study reveals the cellular and spatial organization of BM niches, and offers a novel strategy to dissect the complex organization of whole organs in a systematic manner.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
64.5
作者：
Baryawno, Ninib;Przybylski, Dariusz;Scadden, David T.
通讯作者：
Scadden, David T.
影响因子：
9.3
作者：
Baron M;Veres A;Wolock SL;Faust AL;Gaujoux R;Vetere A;Ryu JH;Wagner BK;Shen-Orr SS;Klein AM;Melton DA;Yanai I
通讯作者：
Yanai I
影响因子：
32.4
作者：
Cordeiro Gomes A;Hara T;Lim VY;Herndler-Brandstetter D;Nevius E;Sugiyama T;Tani-Ichi S;Schlenner S;Richie E;Rodewald HR;Flavell RA;Nagasawa T;Ikuta K;Pereira JP
通讯作者：
Pereira JP
影响因子：
21.3
作者：
Asada N;Kunisaki Y;Pierce H;Wang Z;Fernandez NF;Birbrair A;Ma'ayan A;Frenette PS
通讯作者：
Frenette PS