Genetically encoded lipid-polypeptide hybrid biomaterials that exhibit temperature-triggered hierarchical self-assembly.
Genetically encoded lipid-polypeptide hybrid biomaterials that exhibit temperature-triggered hierarchical self-assembly.
复制标题
DOI：
10.1038/s41557-018-0005-z
复制
发表时间：
2018-05
期刊：
影响因子：
21.8
通讯作者：
Chilkoti A
中科院分区：
文献类型：
--
作者：
Mozhdehi D;Luginbuhl KM;Simon JR;Dzuricky M;Berger R;Varol HS;Huang FC;Buehne KL;Mayne NR;Weitzhandler I;Bonn M;Parekh SH;Chilkoti A
蛋白质的翻译后修饰是生物系统中广泛使用的策略。它扩展了蛋白质组的多样性，并允许定制细胞内蛋白质的功能和定位以及结构蛋白和基质的材料特性。尽管翻译后修饰在生物学中普遍存在，但除少数例外，生物材料合成中翻译后修饰的潜力在很大程度上尚未开发。作为证明通过翻译后修饰创建遗传编码生物杂化材料的可行性的概念验证，本文报告了使用一锅重组表达和翻译后脂化方法生成了一系列三种刺激响应性杂化材料——脂肪酸修饰的弹性蛋白样多肽。这些混合生物材料含有一个两亲结构域，该结构域由翻译后用 C14 烷基链功能化的 β-折叠形成肽组成，并与热响应弹性蛋白样多肽融合。它们在多个长度尺度上表现出温度触发的分层自组装，具有可以在序列水平上控制的不同结构和材料特性。
Post-translational modification of proteins is a strategy widely used in biological systems. It expands the diversity of the proteome, and allows for the tailoring of both function and localization of proteins within cells as well as the material properties of structural proteins and matrices. Despite their ubiquity in biology, with a few exceptions, the potential of post-translational modifications in biomaterials synthesis has remained largely untapped. As a proof-of-concept to demonstrate the feasibility of creating a genetically encoded biohybrid material through post-translational modification, herein we report the generation of a family of three stimulus-responsive hybrid materials — fatty-acid-modified elastin-like polypeptides — using a one-pot recombinant expression and post-translational lipidation methodology. These hybrid biomaterials contain an amphiphilic domain, comprised of a β-sheet-forming peptide that is post-translationally functionalized with a C14 alkyl chain, fused to a thermally responsive elastin-like polypeptide. They exhibit temperature-triggered, hierarchical self-assembly across multiple length scales with varied structure and material properties that can be controlled at the sequence level.
登录
查看更多内容
影响因子：
--
作者：
Hassouneh, Wafa;Christensen, Trine;Chilkoti, Ashutosh
通讯作者：
Chilkoti, Ashutosh
影响因子：
4.9
作者：
Heal, William P.;Wickramasinghe, Sasala R.;Tate, Edward W.
通讯作者：
Tate, Edward W.
影响因子：
28.1
作者：
Luginbuhl, Kelli M.;Schaal, Jeffrey L.;Chilkoti, Ashutosh
通讯作者：
Chilkoti, Ashutosh
DOI：
10.1073/pnas.1214518110
发表时间：
2013-02-19
影响因子：
11.1
作者：
Amiram, Miriam;Luginbuhl, Kelli M.;Chilkoti, Ashutosh
通讯作者：
Chilkoti, Ashutosh
影响因子：
14.9
作者：
Eisenhaber, F;Eisenhaber, B;Wildpaner, M
通讯作者：
Wildpaner, M