Oxidative damage, antioxidant mechanism and gene expression in tomato responding to salinity stress under in vitro conditions and application of iron and zinc oxide nanoparticles on callus induction and plant regeneration.
Oxidative damage, antioxidant mechanism and gene expression in tomato responding to salinity stress under in vitro conditions and application of iron and zinc oxide nanoparticles on callus induction and plant regeneration.
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番茄离体盐胁迫下氧化损伤、抗氧化机制、基因表达及纳米铁、氧化锌在愈伤组织诱导和植株再生中的应用。
DOI：
10.1186/s12870-021-03379-7
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发表时间：
2021-12-16
影响因子：
5.3
通讯作者：
Ebrahimzadeh A
中科院分区：
文献类型：
--
作者：
Aazami MA;Rasouli F;Ebrahimzadeh A
盐度是限制植物生长的最具挑战性的非生物胁迫之一。体外筛选将提高耐盐基因型鉴定的效率和速度。体外分析了四个番茄品种对盐度的响应，以评估幼苗生长、生化和基因表达响应以及纳米锌和铁对愈伤组织诱导和植株再生的影响。结果表明，培养基中盐度胁迫的增加降低了发芽率、芽的鲜重和干重、根长、叶绿素a、b和类胡萝卜素含量、钾和钙含量，另一方面，钠含量增加。与对照相比，MDA 含量（‘Nora’、‘PS-10’、‘Peto’ 和‘Roma’：分别为 1.71、1.78、1.66 和 2.16 倍）、电解质渗漏（‘PS-10’：33.33%；‘Roma’：56.33%）与对照相比，随着盐度为 100 mM 而增加。脯氨酸含量在50mM NaCl中增加（10.8倍）。在盐胁迫下，‘PS-10’品种中的 CAT、SOD、APX、GPX 和 GR 等抗氧化酶活性最高，而‘罗马’品种中这些酶的活性最低。随着盐度强度的增加，AsA和GSH降低，DHA和GSSG增加。不同品种在不同盐度条件下SOD、APX和GR基因的相对表达量存在差异。使用氧化铁纳米粒子观察到最大百分比的愈伤组织诱导，并且使用氧化锌纳米粒子记录最大的再生率。结果表明，“PS-10”等耐盐品种具有更好的渗透调节能力，是未来生产和育种计划的合适候选品种。在盐胁迫下，不同番茄品种使用营养纳米粒子提高了它们的性能。
Salinity is one of the most challenging abiotic stresses restricting the growth of plants. In vitro screening will increase the efficiency and speed of salinity tolerant genotypes identifications. The response of four tomato cultivars under salinity was analyzed in vitro to evaluate the seedlings growth, biochemical, and gene expression responses as well as the effect of nano zinc and iron on callus induction and plant regeneration. The results showed that an increase in salinity stress in the medium decreased the germination percentage, fresh and dry weight of shoot, root length, chlorophyll a, b and carotenoids content, K and Ca content, and on the other hand, Na content was increased. MDA content (‘Nora’, ‘PS-10’, ‘Peto’ and ‘Roma’: 1.71, 1.78, 1.66 and 2.16 folds, respectively), electrolyte leakage (‘PS-10’: 33.33%; ‘Roma’: 56.33%), were increased with salinity of 100 mM compared to control. Proline content was increased in 50 mM NaCl (10.8 fold). The most activity of antioxidant enzymes including CAT, SOD, APX, GPX, and GR was observed in the ‘PS-10’ cultivar, and the lowest activity of these enzymes was observed in ‘Roma’ under salinity stress. The AsA and GSH were decreased and DHA and GSSG were increased with the increased intensity of salinity. The relative expression of SOD, APX, and GR genes varied in different cultivars at different salinity concentrations. The most percentage of callus induction was observed with applying iron oxide nanoparticles, and the most regeneration rate was recorded using zinc oxide nanoparticles. The results showed that salt-tolerant cultivars such as ‘PS-10’ with better osmotic adjustment, are suitable candidates for the future production and breeding programs. The use of nutrient nanoparticles under salinity stress for different tomato cultivars increased their performance.
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影响因子：
6.9
作者：
Cuartero, J;Bolarín, MC;Moreno, V
通讯作者：
Moreno, V
DOI：
10.1139/b96-051
发表时间：
1996-03-01
期刊：
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE
影响因子：
--
作者：
Barka, EA;Audran, JC
通讯作者：
Audran, JC
DOI：
10.3329/jbau.v17i4.44617
发表时间：
2019-01-01
影响因子：
--
作者：
Chakma, Purba;Hossain, Md. Mokter;Rabbani, Md. Golam
通讯作者：
Rabbani, Md. Golam
影响因子：
4.8
作者：
Akbudak, M. Aydin;Filiz, Ertugrul;Kontbay, Kubra
通讯作者：
Kontbay, Kubra
影响因子：
6.7
作者：
Al-Khateeb, Suliman A.;Al-Khateeb, Abdullatif A.;Mohmand, Akbar S.
通讯作者：
Mohmand, Akbar S.