Integrated analysis of long noncoding RNA and coding RNA expression in esophageal squamous cell carcinoma.
Integrated analysis of long noncoding RNA and coding RNA expression in esophageal squamous cell carcinoma.
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DOI：
10.1155/2013/480534
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发表时间：
2013
影响因子：
2.9
通讯作者：
Gu J
中科院分区：
文献类型：
--
作者：
Cao W;Wu W;Shi F;Chen X;Wu L;Yang K;Tian F;Zhu M;Chen G;Wang W;Biddle FG;Gu J
肿瘤的发生是一个复杂的动态生物学过程，包括多个步骤的遗传和表观遗传改变，非编码RNA的异常表达，以及编码基因表达谱的变化。我们把基因组空间中这些微扰的集合称为“癌症启动体”。长非编码RNA(LncRNAs)在基因组中广泛转录，在染色质重塑和基因表达中具有关键的调节功能。在发育和疾病状态下，包括癌症在内，已经观察到LncRNAs表达的时空差异。已有研究表明，在肿瘤发生发展过程中，存在一些调控异常的LncRNAs，但LncRNAs在肿瘤发生发展过程中的作用仍不十分清楚，尤其是在食管鳞癌中。我们对食管鳞癌和配对的邻近非肿瘤正常组织中的lncRNAs和编码RNAs的表达进行了全基因组筛选。我们鉴定了在ESCC中相对于其匹配的正常组织的差异表达的LncRNAs和编码RNAs，并用聚合酶链式反应分析验证了结果。此外，我们还鉴定了在食管鳞癌中与差异表达的编码RNA共定位和共表达的差异表达的lncRNAs，结果表明，lncRNAs与邻近的编码基因之间存在潜在的相互作用，影响乙醚类脂代谢，这种相互作用可能有助于ESCC的发展。这些数据为食管鳞癌潜在的新的基因组生物标记物提供了令人信服的证据。
Tumorigenesis is a complex dynamic biological process that includes multiple steps of genetic and epigenetic alterations, aberrant expression of noncoding RNA, and changes in the expression profiles of coding genes. We call the collection of those perturbations in genome space the “cancer initiatome.” Long noncoding RNAs (lncRNAs) are pervasively transcribed in the genome and they have key regulatory functions in chromatin remodeling and gene expression. Spatiotemporal variation in the expression of lncRNAs has been observed in development and disease states, including cancer. A few dysregulated lncRNAs have been studied in cancers, but the role of lncRNAs in the cancer initiatome remains largely unknown, especially in esophageal squamous cell carcinoma (ESCC). We conducted a genome-wide screen of the expression of lncRNAs and coding RNAs from ESCC and matched adjacent nonneoplastic normal tissues. We identified differentially expressed lncRNAs and coding RNAs in ESCC relative to their matched normal tissue counterparts and validated the result using polymerase chain reaction analysis. Furthermore, we identified differentially expressed lncRNAs that are co-located and co-expressed with differentially expressed coding RNAs in ESCC and the results point to a potential interaction between lncRNAs and neighboring coding genes that affect ether lipid metabolism, and the interaction may contribute to the development of ESCC. These data provide compelling evidence for a potential novel genomic biomarker of esophageal squamous cell cancer.
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