Effective elimination of liver cancer stem-like cells by CD90 antibody targeted thermosensitive magnetoliposomes.
Effective elimination of liver cancer stem-like cells by CD90 antibody targeted thermosensitive magnetoliposomes.
复制标题
CD90抗体靶向热敏磁脂质体有效消除肝癌干细胞样细胞
DOI：
10.18632/oncotarget.9116
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发表时间：
2016-06-14
期刊：
影响因子：
--
通讯作者：
Tang SQ
中科院分区：
文献类型：
--
作者：
Yang R;An LY;Miao QF;Li FM;Han Y;Wang HX;Liu DP;Chen R;Tang SQ
目的探讨负载磁性氧化铁(Fe3O4)的温敏磁性脂质体(TMS)和抗癌干细胞标志物CD90(CD90@TMS)对CD90+肝癌干细胞的靶向和杀伤作用。方法以肝细胞癌细胞系HuH7为材料，采用磁激活细胞分选法分离CD90+LCSCs。对CD90@TMS进行了鉴定，并测定了其靶向CD90+LC SCs的能力。通过实验研究CD90@TMS联合磁热治疗能否有效消除CD90+LC SCs。结果成功分离出具有干细胞特性的CD90+LCSCs。我们还成功地制备了CD90@TMS，其平均直径为130±4.6 nm，几乎是球形的，并确定了磁性氧化铁可以被掺入并保持超顺磁性响应。与TMS相比，CD90@TMS具有良好的靶向性，在体内外对CD90+LCSCs的抑制作用增强。结论CD90@TMS可用于抗癌药物的控释和靶向给药，为肝癌的治疗提供了一种有前景的选择。
Aim To investigate the use of thermosensitive magnetoliposomes (TMs) loaded with magnetic iron oxide (Fe3O4) and the anti-cancer stem cell marker CD90 (CD90@TMs) to target and kill CD90+ liver cancer stem cells (LCSCs). Methods The hepatocellular carcinoma cell line Huh7 was used to separate CD90+ LCSCs by magnetic-activated cell sorting. CD90@TMs was characterized and their ability to target CD90+ LCSCs was determined. Experiments were used to investigate whether CD90@TMs combined with magnetic hyperthermia could effectively eliminate CD90+ LCSCs. Results The present study demonstrated that CD90+ LCSCs with stem cells properties were successfully isolated. We also successfully prepared CD90@TMs that was almost spherical and uniform with an average diameter of 130±4.6 nm and determined that magnetic iron oxide could be incorporated and retained a superparamagnetic response. CD90@TMs showed good targeting and increased inhibition of CD90+ LCSCs in vitro and in vivo compared to TMs. Conclusion CD90@TMs can be used for controlled and targeted delivery of anticancer drugs, which may offer a promising alternative for HCC therapy.
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影响因子：
--
作者：
Iacovelli S;Ricciardi MR;Allegretti M;Mirabilii S;Licchetta R;Bergamo P;Rinaldo C;Zeuner A;Foà R;Milella M;McCubrey JA;Martelli AM;Tafuri A
通讯作者：
Tafuri A
影响因子：
8
作者：
Kneidl B;Peller M;Winter G;Lindner LH;Hossann M
通讯作者：
Hossann M
影响因子：
17.1
作者：
Atkinson RL;Zhang M;Diagaradjane P;Peddibhotla S;Contreras A;Hilsenbeck SG;Woodward WA;Krishnan S;Chang JC;Rosen JM
通讯作者：
Rosen JM
影响因子：
14
作者：
Burke, Andrew R.;Singh, Ravi N.;Carroll, David L.;Wood, James C. S.;D'Agostino, Ralph B., Jr.;Ajayan, Pulickel M.;Torti, Frank M.;Torti, Suzy V.
通讯作者：
Torti, Suzy V.
影响因子：
8
作者：
Franke K;Kettering M;Lange K;Kaiser WA;Hilger I
通讯作者：
Hilger I