Peripheral nerve growth within a hydrogel microchannel scaffold supported by a kink-resistant conduit.
Peripheral nerve growth within a hydrogel microchannel scaffold supported by a kink-resistant conduit.
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DOI：
10.1002/jbm.a.36186
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发表时间：
2017-12
期刊：
影响因子：
--
通讯作者：
Sakamoto JS
中科院分区：
文献类型：
--
作者：
Shahriari D;Shibayama M;Lynam DA;Wolf KJ;Kubota G;Koffler JY;Tuszynski MH;Campana WM;Sakamoto JS
在几毫米长的神经间隙中的神经修复通常需要介入技术。微通道支架已被证明可有效地桥接周围神经系统（PNS）中的神经间隙和引导轴突。尽管如此，以这种长度规模制造微通道支架仍然是一个挑战和/或耗时且麻烦。在这项工作中，一个简单的计算机辅助微钻孔技术被用来制造10毫米长的琼脂糖支架组成的300 μ m的微通道和85 μ m厚的壁在不到一个小时。然而，单独的琼脂糖支架没有表现出足够的刚度和完整性来承受植入和植入期间的机械应力。为了提供机械支撑并使其能够被植入，制造聚己内酯（PCL）导管并将琼脂糖支架放置在内部。开发了一种改进的盐浸技术，以在PCL导管中引入互连的孔隙率，以允许调整机械性能，例如弹性模量和失效应变。结果表明，PCL导管在稳定大鼠坐骨神经10 mm长的间隙中的琼脂糖支架至少8周是有效的。在不使用生长因子的情况下，在微通道支架内观察到强大的轴突进入和雪旺细胞渗透。
Nerve repair in several mm-long nerve gaps often requires an interventional technology. Microchannel scaffolds have proven effective for bridging nerve gaps and guiding axons in the peripheral nervous system (PNS). Nonetheless, fabricating microchannel scaffolds at this length scale remains a challenge and/or is time consuming and cumbersome. In this work, a simple computer-aided microdrilling technique was used to fabricate 10 mm-long agarose scaffolds consisting of 300 μm-microchannels and 85 μm-thick walls in less than an hour. The agarose scaffolds alone, however, did not exhibit adequate stiffness and integrity to withstand the mechanical stresses during implantation and suturing. To provide mechanical support and enable suturing, poly caprolactone (PCL) conduits were fabricated and agarose scaffolds were placed inside. A modified salt-leaching technique was developed to introduce interconnected porosity in PCL conduits to allow for tuning of the mechanical properties such as elastic modulus and strain to failure. It was shown that the PCL conduits were effective in stabilizing the agarose scaffolds in 10 mm-long sciatic nerve gaps of rats for at least 8 weeks. Robust axon ingress and Schwann cell penetration were observed within the microchannel scaffolds without using growth factors.
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影响因子：
14
作者：
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