Cloning and sequence analysis of the nucleocapsid gene of porcine epidemic diarrhea virus Chinju99.
Cloning and sequence analysis of the nucleocapsid gene of porcine epidemic diarrhea virus Chinju99.
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猪腹泻病毒chinju99的核蛋白基因基因的克隆和序列分析。
DOI：
10.1023/a:1023447732567
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发表时间：
2003
期刊：
影响因子：
1.6
通讯作者：
Yeo SG
中科院分区：
文献类型：
--
作者：
Lee HK;Yeo SG
对韩国济州分离的猪流行性腹泻病毒(PEDV)Chinju99株核衣壳蛋白(N)基因进行了克隆和测序，为基因工程诊断试剂的研制奠定了基础。此外，还与CV777和BR1/87进行了核苷酸序列和氨基酸序列分析。编码锦菊99株N基因开放阅读框(ORF)的核苷酸序列全长1326个碱基，编码441个氨基酸，预测MR为49 kDa。由405个腺嘌呤(30.5%)、293个胞嘧啶(22.1%)、334个鸟嘌呤(25.2%)和294个胸腺嘧啶(22.2%)残基组成。与CV777和BR1/87的核苷酸同源性分别为96.5%和96.4%。Chinju99N蛋白与BR1/87和CV777的氨基酸同源性分别为96.8%。该氨基酸序列包含7个潜在的苏氨酸(T)或丝氨酸(S)连接的磷酸化位点，分别被蛋白激酶C和酪蛋白激酶II所激活。
The nucleocapsid (N) gene of the porcine epidemic diarrhea virus (PEDV) Chinju99 which was previously isolated in Chinju, Korea was cloned and sequenced to establish the information for the development of genetically engineered diagnostic reagents. Also, sequences of the nucleotides and deduced amino acids of the Chinju99 N gene were analyzed by alignment with those of CV777 and Br1/87. The nucleotide sequence encoding the entire N gene open reading frame (ORF) of Chinju99 was 1326 bases long and encoded a protein of 441 amino acids with predicted M r of 49 kDa. It consisted of 405 adenine (30.5%), 293 cytosine (22.1%), 334 guanines (25.2%) and 294 thymines (22.2%) residues. The Chinju99 N ORF nucleotide sequence was 96.5% and 96.4% homologous with that of the CV777 and Br1/87, respectively. The Chinju99 N protein revealed 96.8% amino acid identity with that of Br1/87 and CV777, respectively. The amino acid sequence contained seven potential sites for threonine (T)- or serine (S)-linked phosphorylation by each protein kinase C and casein kinase II.
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影响因子：
1.6
作者：
UTIGER, A;TOBLER, K;ACKERMANN, M
通讯作者：
ACKERMANN, M
影响因子：
3.7
作者：
DUARTE, M;TOBLER, K;LAUDE, H
通讯作者：
LAUDE, H
影响因子：
5.7
作者：
PARKER, RG
通讯作者：
PARKER, RG
影响因子：
3.3
作者：
Knuchel M;Ackermann M;Müller HK;Kihm U
通讯作者：
Kihm U
影响因子：
3.8
作者：
DUARTE, M;LAUDE, H
通讯作者：
LAUDE, H