Use of transcriptomic profiling to identify candidate genes involved in Polyporus umbellatus sclerotial formation affected by oxalic acid.
Use of transcriptomic profiling to identify candidate genes involved in Polyporus umbellatus sclerotial formation affected by oxalic acid.
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使用转录组分析来鉴定参与受草酸影响的猪苓菌核形成的候选基因
DOI：
10.1038/s41598-021-96740-7
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发表时间：
2021-08-30
影响因子：
4.6
通讯作者：
Guo SX
中科院分区：
文献类型：
--
作者：
Xing YM;Li B;Zeng X;Zhou LS;Lee TS;Lee MW;Chen XM;Guo SX
猪苓是一种珍贵的药用真菌。草酸影响菌核形成，菌核比菌丝体具有更多的药用化合物。在这项研究中，在真菌暴露于不同浓度的草酸后，分析了猪苓的转录组。与对照组（No OA）相比，低草酸（Low OA）组编码一系列氧化酶的差异表达基因（DEG）表达上调，还原酶表达下调，而高草酸（High OA）组则相反。采用目测法检测猪苓菌丝体中活性氧（ROS）的含量，采用非侵入性微测技术（NMT）测定菌丝体中Ca2+和H2O2的通量。低OA组中的菌核生物量增加了66%，然而，在高OA组中没有形成菌核。ROS荧光强度在低OA组中显著增加，但在高OA组中显著降低。低OA组Ca2+和H2O2内流显著增加，而高OA组H2O2表现出外流。在低OA组中形成了更高水平的氧化应激。研究了不同浓度的草酸对猪苓菌核形成的影响。
Polyporus umbellatus is a precious medicinal fungus. Oxalic acid was observed to affect sclerotial formation and sclerotia possessed more medicinal compounds than mycelia. In this study, the transcriptome of P. umbellatus was analysed after the fungus was exposed to various concentrations of oxalic acid. The differentially expressed genes (DEGs) encoding a series of oxidases were upregulated, and reductases were downregulated, in the low-oxalic-acid (Low OA) group compared to the control (No OA) group, while the opposite phenomenon was observed in the high-oxalic-acid (High OA) group. The detection of reactive oxygen species (ROS) in P. umbellatus mycelia was performed visually, and Ca2+ and H2O2 fluxes were measured using non-invasive micro-test technology (NMT). The sclerotial biomass in the Low OA group increased by 66%, however, no sclerotia formed in the High OA group. The ROS fluorescence intensity increased significantly in the Low OA group but decreased considerably in the High OA group. Ca2+ and H2O2 influx significantly increased in the Low OA group, while H2O2 exhibited efflux in the High OA group. A higher level of oxidative stress formed in the Low OA group. Different concentrations of oxalic acid were determined to affect P. umbellatus sclerotial formation in different ways.
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影响因子：
--
作者：
Georgiou, CD
通讯作者：
Georgiou, CD
拟南芥生态型的比较分析揭示了油菜素类固醇在根向水性中的作用
影响因子：
7.4
作者：
Miao, Rui;Wang, Meng;Xu, Weifeng
通讯作者：
Xu, Weifeng
蜜环菌感染猪苓防御反应相关基因的转录组分析
影响因子：
4.6
作者：
Liu MM;Xing YM;Zhang DW;Guo SX
通讯作者：
Guo SX
影响因子：
4.3
作者：
Di Palma, Andres Arruebarrena;Di Fino, Luciano M.;Laxalt, Ana M.
通讯作者：
Laxalt, Ana M.
DOI：
10.1083/jcb.139.6.1419
发表时间：
1997-12-15
期刊：
The Journal of cell biology
影响因子：
--
作者：
Waterham HR;Russell KA;Vries Y;Cregg JM
通讯作者：
Cregg JM