Interface-directed self-assembly of cell-laden microgels.
Interface-directed self-assembly of cell-laden microgels.
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DOI：
10.1002/smll.200902326
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发表时间：
2010-04-23
期刊：
影响因子：
13.3
通讯作者：
Khademhosseini, Ali
中科院分区：
文献类型：
--
作者：
Zamanian, Behnam;Masaeli, Mandokht;Nichol, Jason W.;Khabiry, Masoud;Hancock, Matthew J.;Bae, Hojae;Khademhosseini, Ali
载细胞水凝胶在制造工程化组织方面显示出巨大的前景。然而，这些系统的一个主要缺点是无法在生物相关的长度尺度上制造具有受控微尺度特征的结构。在这里，我们展示了一种通过形状控制的微凝胶的组装来快速创建厘米级、细胞负载的水凝胶的方法。这是通过使用一种利用疏水溶液的液-气界面来驱动微凝胶组装的方法实现的。将特定形状的载细胞微凝胶随机放置在高密度疏水溶液的表面，诱导聚集，然后交联成宏观组织状结构。得到的组件是由紧密堆积的有序微凝胶单元组成的细胞加载的水凝胶片。此外，一种分层方法创建了复杂的多凝胶构建块，然后将这些构建块组装成组织，并对细胞分布进行精确的空间控制。这些数据表明，气液界面上的力可以用来自组装空间可控的、共培养的组织状结构。
Cell-laden hydrogels show great promise for creating engineered tissues. However, a major shortcoming with these systems has been the inability to fabricate structures with controlled microscale features on a biologically relevant length scale. Here we demonstrate a rapid method for creating centimeter-scale, cell-laden hydrogels through the assembly of shape-controlled microgels. This was achieved by using an approach that uses the liquid-air interface of a hydrophobic solution to drive the assembly of microgels. Cell-laden microgels of specific shapes were randomly placed on the surface of a high density, hydrophobic solution and induced to aggregate and were subsequently crosslinked into macroscale tissue-like structures. The resultant assemblies were cell-laden hydrogel sheets consisting of tightly-packed ordered microgel units. In addition, a hierarchical approach created complex multi-gel building blocks which were then assembled into tissues with precise spatial control over the cell distribution. These data demonstrate that forces at an air-liquid interface can be used to self-assemble spatially controllable, co-cultured tissue-like structures.
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影响因子：
14
作者：
Mann, BK;Gobin, AS;West, JL
通讯作者：
West, JL
DOI：
10.1073/pnas.0801866105
发表时间：
2008-07-15
影响因子：
11.1
作者：
Du, Yanan;Lo, Edward;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
DOI：
10.1021/je060199g
发表时间：
2006-09-14
期刊：
JOURNAL OF CHEMICAL AND ENGINEERING DATA
影响因子：
--
作者：
Freire, Mara G.;Carvalho, Pedro J.;Coutinho, Joao A. P.
通讯作者：
Coutinho, Joao A. P.
影响因子：
--
作者：
Benton JA;DeForest CA;Vivekanandan V;Anseth KS
通讯作者：
Anseth KS
影响因子：
4.8
作者：
Gobin, AS;West, JL
通讯作者：
West, JL