Induced pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures.
Induced pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures.
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DOI：
10.1016/j.stem.2009.06.008
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发表时间：
2009-07-02
期刊：
影响因子：
23.9
通讯作者：
Lowry WE
中科院分区：
文献类型：
--
作者：
Chin MH;Mason MJ;Xie W;Volinia S;Singer M;Peterson C;Ambartsumyan G;Aimiuwu O;Richter L;Zhang J;Khvorostov I;Ott V;Grunstein M;Lavon N;Benvenisty N;Croce CM;Clark AT;Baxter T;Pyle AD;Teitell MA;Pelegrini M;Plath K;Lowry WE
诱导多能干细胞（iPSC）表面上看起来与胚胎干细胞（ESC）无法区分。对小鼠和人类ESC和iPSC的基因表达谱的研究表明，虽然iPSC与它们的胚胎对应物非常相似，但iPSC中出现了重复的基因表达特征，无论它们的来源或产生它们的方法如何。在延长培养后，hiPSC采用与hESC更相似的基因表达谱;然而，它们仍然保留了从hESC延伸到miRNA表达的独特基因表达特征。全基因组数据表明，iPSC标签基因表达差异是由于重编程因子的差异启动子结合。高分辨率阵列分析表明，没有常见的特定亚核型改变，需要重编程和重编程不会导致基因组不稳定性。总之，这些数据表明，iPSC应被视为多能细胞的独特亚型。
Induced pluripotent stem cells (iPSCs) outwardly appear to be indistinguishable from embryonic stem cells (ESCs). A study of gene expression profiles of mouse and human ESCs and iPSCs suggests that, while iPSCs are quite similar to their embryonic counterparts, a recurrent gene expression signature appears in iPSCs regardless of their origin or the method by which they were generated. Upon extended culture, hiPSCs adopt a gene expression profile more similar to hESCs; however, they still retain a gene expression signature unique from hESCs that extends to miRNA expression. Genome-wide data suggested that the iPSC signature gene expression differences are due to differential promoter binding by the reprogramming factors. High-resolution array profiling demonstrated that there is no common specific subkaryotypic alteration that is required for reprogramming and that reprogramming does not lead to genomic instability. Together, these data suggest that iPSCs should be considered a unique subtype of pluripotent cell.
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影响因子：
5.2
作者：
Park, Tae Sub;Galic, Zoran;Conway, Anne E.;Lindgren, Anne;van Handel, Benjamin J.;Magnusson, Mattias;Richter, Laura;Teitell, Michael A.;Mikkola, Hanna K. A.;Lowry, William E.;Plath, Kathrin;Clark, Amander T.
通讯作者：
Clark, Amander T.
影响因子：
64.8
作者：
Park, In-Hyun;Zhao, Rui;Daley, George Q.
通讯作者：
Daley, George Q.
影响因子：
64.5
作者：
Soldner F;Hockemeyer D;Beard C;Gao Q;Bell GW;Cook EG;Hargus G;Blak A;Cooper O;Mitalipova M;Isacson O;Jaenisch R
通讯作者：
Jaenisch R
影响因子：
4.8
作者：
Markoulaki, Styliani;Meissner, Alexander;Jaenisch, Rudolf
通讯作者：
Jaenisch, Rudolf
DOI：
10.1073/pnas.0711983105
发表时间：
2008-02-26
影响因子：
11.1
作者：
Lowry, W. E.;Richter, L.;Plath, K.
通讯作者：
Plath, K.